DNA-free genome editing: past, present and future

J Metje-Sprink, J Menz, D Modrzejewski… - Frontiers in Plant …, 2019 - frontiersin.org
Genome Editing using engineered endonuclease (GEEN) systems rapidly took over the field
of plant science and plant breeding. So far, Genome Editing techniques have been applied …

Technological development and application of plant genetic transformation

W Su, M Xu, Y Radani, L Yang - International Journal of Molecular …, 2023 - mdpi.com
Genetic transformation is an important strategy for enhancing plant biomass or resistance in
response to adverse environments and population growth by imparting desirable genetic …

Oil palm natural diversity and the potential for yield improvement

E Barcelos, SA Rios, RNV Cunha, R Lopes… - Frontiers in plant …, 2015 - frontiersin.org
African oil palm has the highest productivity amongst cultivated oleaginous crops. Species
can constitute a single crop capable to fulfill the growing global demand for vegetable oils …

Current status and perspectives of genome editing technology for microalgae

S Jeon, JM Lim, HG Lee, SE Shin, NK Kang… - Biotechnology for …, 2017 - Springer
Genome editing techniques are critical for manipulating genes not only to investigate their
functions in biology but also to improve traits for genetic engineering in biotechnology …

Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2

S Biswas, NJ Wahl, MJ Thomson, JM Cason… - International Journal of …, 2022 - mdpi.com
The cultivated peanut (Arachis hypogaea L.) is a legume consumed worldwide in the form of
oil, nuts, peanut butter, and candy. Improving peanut production and nutrition will require …

Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for …

K Nanjareddy, MK Arthikala, L Blanco, ES Arellano… - BMC …, 2016 - Springer
Background Phaseolus vulgaris is one of the most extensively studied model legumes in the
world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and …

Highly efficient leaf base protoplast isolation and transient expression systems for orchids and other important monocot crops

R Ren, J Gao, D Yin, K Li, C Lu, S Ahmad… - Frontiers in plant …, 2021 - frontiersin.org
Versatile protoplast platforms greatly facilitate the development of modern botany. However,
efficient protoplast-based systems are still challenging for numerous horticultural plants and …

Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro− transfection

S Subburaj, SZ Agapito-Tenfen - Frontiers in Plant Science, 2023 - frontiersin.org
The soybean (Glycine max L.) is an important crop with high agronomic value. The
improvement of agronomic traits through gene editing techniques has broad application …

Genome editing in crop improvement: present scenario and future prospects

GS Miglani - Journal of Crop Improvement, 2017 - Taylor & Francis
Genome editing refers to a process by which a specific chromosomal sequence is changed.
The edited chromosomal sequence may comprise an insertion of at least one nucleotide, a …

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids

R Ren, J Gao, C Lu, Y Wei, J Jin, SM Wong… - International journal of …, 2020 - mdpi.com
Protoplast systems have been proven powerful tools in modern plant biology. However,
successful preparation of abundant viable protoplasts remains a challenge for Cymbidium …