This unit outlines the steps required to prepare a sample for MS analysis following protein
separation or enrichment by gel electrophoresis, liquid chromatography, and affinity capture …

Mass spectrometry is the tool of choice to investigate protein phosphorylation, which plays a
vital role in cell regulation and diseases such as cancer. However, low abundances of …

There are likely more than 500000 potential phosphorylation sites in a cellular proteome.
This dynamic phosphorylation is under tight control of a variety of kinases and …

Root hairs are single hair-forming cells on roots that function to increase root surface area,
enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role …

Phosphoproteomics involves identification of phosphoproteins, precise mapping, and
quantification of phosphorylation sites, and eventually, revealing their biological function. In …

Nuclear receptors (NRs) are molecular transducers of endocrine and dietary signals
allowing tissues to adapt their transcriptional responses to endogenous or exogenous cues …

Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced
recently for phosphopeptide enrichment. Here we compared ERLIC with the well …

The emergence of 'shotgun proteomics' has paved the way for high-throughput proteome
analysis, by which thousands of proteins can be identified simultaneously from complex …

Activation of signal transduction by the receptor tyrosine kinase, fibroblast growth factor
receptor (FGFR), results in a cascade of protein− protein interactions that rely on the …

We developed a novel approach to enlarge phosphoproteome coverage by selective elution
depending on the number of phosphoryl group of peptides from a single titanium dioxide …