The use of monoclonal antibodies as therapeutics requires optimizing several of their key
attributes. These include binding affinity and specificity, folding stability, solubility …

Phage display involves the expression of selected proteins on the surface of filamentous
phage through fusion with phage coat protein, with the genetic sequence packaged within …

Phosphorylation is often used to promote protein ubiquitylation, yet we rarely understand
quantitatively how ligase activation and ubiquitin (UB) chain assembly are integrated with …

Antibodies are key reagents in biology and medicine, but commercial sources are rarely
recombinant and thus do not provide a permanent and renewable resource. Here, we …

The book provides a comprehensive view of the impact and promise of phage display in
drug discovery and biotechnology. Building on the success of its previous edition, the book …

Multiplex detection of protein post‐translational modifications (PTMs), especially at point‐of‐
care, is of great significance in cancer diagnosis. Herein, we report a machine learning …

We have developed a novel biosensor for kinases that is based on a kinase-responsive
polymer hydrogel, which enables label-free screening of kinase activity via changes in …

Phage display is a powerful approach for evolving proteins and peptides with new functions,
but the properties of the molecules that can be evolved are limited by the chemical diversity …

Antibody phage display libraries combined with high-throughput selections have recently
demonstrated tremendous promise to create the next generation of renewable, recombinant …

Highlights•Synthetic Abs are designed using two distinct design philosophies.•Mammalian
display of synthetic Abs can provide improved functional assessment.•Diversified synthetic …