A technical review and guide to RNA fluorescence in situ hybridization
RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target
messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations …
messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations …
Recent advances in single extracellular vesicle detection methods
SU Wang, A Khan, R Huang, S Ye, K Di… - Biosensors and …, 2020 - Elsevier
Extracellular vesicles (EVs) are secreted by a variety of cells. They are known for their
pertinent role in intercellular communication, and participation in different pathological …
pertinent role in intercellular communication, and participation in different pathological …
Long Noncoding RNA MALAT1 and Regulation of the Antioxidant Defense System in Diabetic Retinopathy
R Radhakrishnan, RA Kowluru - Diabetes, 2021 - Am Diabetes Assoc
The retina experiences increased oxidative stress in diabetes, and the transcriptional activity
of Nrf2, which is critical in regulating many antioxidant genes, is decreased. The nuclear …
of Nrf2, which is critical in regulating many antioxidant genes, is decreased. The nuclear …
RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection
The RIG-I like receptors (RLRs) RIG-I and MDA5 are cytosolic RNA helicases best
characterized as restriction factors for RNA viruses. However, evidence suggests RLRs …
characterized as restriction factors for RNA viruses. However, evidence suggests RLRs …
S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo
HIV-1 eradication is hindered by viral persistence in cell reservoirs, established not only in
circulatory CD4+ T-cells but also in tissue-resident macrophages. The nature of macrophage …
circulatory CD4+ T-cells but also in tissue-resident macrophages. The nature of macrophage …
Transcriptional heterogeneity of beta cells in the intact pancreas
Pancreatic beta cells have been shown to be heterogeneous at multiple levels. However,
spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging …
spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging …
MicroRNA expression profile analysis in sperm reveals hsa-mir-191 as an auspicious omen of in vitro fertilization
H Xu, X Wang, Z Wang, J Li, Z Xu, M Miao, G Chen… - Bmc Genomics, 2020 - Springer
Background MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important
roles in many physiological processes by regulating gene expression. Previous studies …
roles in many physiological processes by regulating gene expression. Previous studies …
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics
Rare cells have an important role in development and disease, and methods for isolating
and studying cell subsets are therefore an essential part of biology research. Such methods …
and studying cell subsets are therefore an essential part of biology research. Such methods …
Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
HY Wang, JY Chen, Y Li, X Zhang, X Liu, Y Lu… - Nature …, 2024 - nature.com
Elucidating cellular architecture and cell-type evolution across species is central to
understanding immune system function and susceptibility to disease. Adaptive immunity is a …
understanding immune system function and susceptibility to disease. Adaptive immunity is a …
[HTML][HTML] Regulatory guidelines and preclinical tools to study the biodistribution of RNA therapeutics
P Vervaeke, SE Borgos, NN Sanders… - Advanced Drug Delivery …, 2022 - Elsevier
The success of the messenger RNA-based COVID-19 vaccines of Moderna and
Pfizer/BioNTech marks the beginning of a new chapter in modern medicine. However, the …
Pfizer/BioNTech marks the beginning of a new chapter in modern medicine. However, the …