Time-resolved photothermal methods: accessing time-resolved thermodynamics of photoinduced processes in chemistry and biology

T Gensch, C Viappiani - Photochemical & photobiological sciences, 2003 - pubs.rsc.org
Photothermal methods are currently being employed in a variety of research areas, ranging
from materials science to environmental monitoring. Despite the common term which they …

[HTML][HTML] Fast events in protein folding: structural volume changes accompanying the early events in the N→ I transition of apomyoglobin induced by ultrafast pH jump

S Abbruzzetti, E Crema, L Masino, A Vecli… - Biophysical journal, 2000 - cell.com
Ultrafast, laser-induced pH jump with time-resolved photoacoustic detection has been used
to investigate the early protonation steps leading to the formation of the compact acid …

Structural Volume Changes upon Photoexcitation of Porphyrins: Role of the Nitrogen− Water Interactions

T Gensch, C Viappiani… - Journal of the American …, 1999 - ACS Publications
The molecular structural volume change (as determined by laser-induced optoacoustics), Δ
VR∼− 18 Å3, accompanying triplet state formation of free base 5, 10, 15, 20-tetrakis-(4 …

Time-resolved methods in Biophysics. 2. Monitoring haem proteins at work with nanosecond laser flash photolysis

S Abbruzzetti, S Bruno, S Faggiano, E Grandi… - Photochemical & …, 2006 - Springer
Haem proteins have long been the most studied proteins in biophysics, and have become
paradigms for the characterization of fundamental biomolecular processes as ligand binding …

Kinetics of acid-induced spectral changes in the GFPmut2 chromophore

S Abbruzzetti, E Grandi, C Viappiani… - Journal of the …, 2005 - ACS Publications
We have used a nanosecond pH-jump technique, coupled with simultaneous transient
absorption and fluorescence emission detection, to characterize the dynamics of the acid …

[HTML][HTML] Kinetics of local helix formation in poly-L-glutamic acid studied by time-resolved photoacoustics: neutralization reactions of carboxylates in aqueous solutions …

S Abbruzzetti, C Viappiani, JR Small, LJ Libertini… - Biophysical …, 2000 - cell.com
Photoactivatable caged protons have been used to trigger proton transfer reactions in
aqueous solutions of acetate, glutamate, and poly-L-glutamic acid, and the volumetric and …

Folding Kinetics and Volume Variation of the β-Hairpin Peptide Chignolin upon Ultrafast pH-Jumps

D Amado, OA Chaves, PF Cruz… - The Journal of …, 2024 - ACS Publications
In-depth characterization of fundamental folding steps of small model peptides is crucial for
a better understanding of the folding mechanisms of more complex biomacromolecules. We …

Acid-induced unfolding of myoglobin triggered by a laser pH jump method

S Abbruzzetti, S Sottini, C Viappiani… - Photochemical & …, 2006 - Springer
Abstract Using 1-(2-nitrophenyl) ethyl sulfate (caged sulfate) as a photoactivatable caged
proton, we could induce complete acid unfolding of myoglobin with a single nanosecond …

Photochemical reagents for the study of metalloproteins by flash photolysis

FI Rosell, AG Mauk - Coordination Chemistry Reviews, 2011 - Elsevier
Flash photolysis has been used extensively in the advancement of our understanding of the
electron transfer reactivity of metalloproteins and in investigation of the kinetic complexities …

Kinetics of proton release after flash photolysis of 1-(2-nitrophenyl) ethyl sulfate (caged sulfate) in aqueous solution

S Abbruzzetti, S Sottini, C Viappiani… - Journal of the American …, 2005 - ACS Publications
The kinetics of proton release after laser photolysis of 1-(2-nitrophenyl) ethyl sulfate (caged
sulfate) have been characterized by time-resolved absorbance and photoacoustic methods …