Bottom-up proteomics: advancements in sample preparation

VA Duong, H Lee - International journal of molecular sciences, 2023 - mdpi.com
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …

Making proteomics data accessible and reusable: current state of proteomics databases and repositories

Y Perez‐Riverol, E Alpi, R Wang, H Hermjakob… - …, 2015 - Wiley Online Library
Compared to other data‐intensive disciplines such as genomics, public deposition and
storage of MS‐based proteomics, data are still less developed due to, among other reasons …

Review of three-dimensional liquid chromatography platforms for bottom-up proteomics

VA Duong, JM Park, H Lee - International Journal of Molecular Sciences, 2020 - mdpi.com
Proteomics is a large-scale study of proteins, aiming at the description and characterization
of all expressed proteins in biological systems. The expressed proteins are typically highly …

Marine organisms as potential sources of bioactive peptides that inhibit the activity of angiotensin I-converting enzyme: a review

DY Pujiastuti, MN Ghoyatul Amin, MA Alamsjah, JL Hsu - Molecules, 2019 - mdpi.com
Angiotensin I-converting enzyme (ACE) is a paramount therapeutic target to treat
hypertension. ACE inhibitory peptides derived from food protein sources are regarded as …

In-depth analysis of protein inference algorithms using multiple search engines and well-defined metrics

E Audain, J Uszkoreit, T Sachsenberg, J Pfeuffer… - Journal of …, 2017 - Elsevier
In mass spectrometry-based shotgun proteomics, protein identifications are usually the
desired result. However, most of the analytical methods are based on the identification of …

Qualitative and quantitative characterization of protein biotherapeutics with liquid chromatography mass spectrometry

M Qu, B An, S Shen, M Zhang, X Shen… - Mass Spectrometry …, 2017 - Wiley Online Library
In the last decade, the advancement of liquid chromatography mass spectrometry (LC/MS)
techniques has enabled their broad application in protein characterization, both …

Accurate quantitative proteomic analyses using metabolic labeling and high field asymmetric waveform ion mobility spectrometry (FAIMS)

S Pfammatter, E Bonneil, FP McManus… - Journal of proteome …, 2019 - ACS Publications
Stable isotope labeling by amino acids in cell culture (SILAC) is routinely used to profile
changes in protein and peptide abundance across different experimental paradigms. As with …

3D HPLC-MS with reversed-phase separation functionality in all three dimensions for large-scale bottom-up proteomics and peptide retention data collection

V Spicer, P Ezzati, H Neustaeter, RC Beavis… - Analytical …, 2016 - ACS Publications
The growing complexity of proteomics samples and the desire for deeper analysis drive the
development of both better MS instrument and advanced multidimensional separation …

First Report of Screening of Novel Angiotensin-I Converting Enzyme Inhibitory Peptides Derived from the Red Alga Acrochaetium sp.

S Windarto, MC Lee, H Nursyam, JL Hsu - Marine Biotechnology, 2022 - Springer
ACE inhibitors generated from food proteins have recently become the most well-known
subclass of bioactive peptides, and their bio-functionality can be a potential alternative to …

[HTML][HTML] Spermine and spermidine reversed age-related cardiac deterioration in rats

H Zhang, J Wang, L Li, N Chai, Y Chen, F Wu… - Oncotarget, 2017 - ncbi.nlm.nih.gov
Aging is the most important risk factor for cardiovascular disease (CVD). Slowing or
reversing the physiological impact of heart aging may reduce morbidity and mortality …