CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

The past 25 years of genomics research first revealed which genes are encoded by the
human genome and then a detailed catalogue of human genome variation associated with …

Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …

Video-based screening of pooled libraries is a powerful approach for directed evolution of
biosensors because it enables selection along multiple dimensions simultaneously from …

Comprehensively charting the biologically causal circuits that govern the phenotypic space
of human cells has often been viewed as an insurmountable challenge. However, in the last …

The understanding of lysosomes has come a long way since the initial discovery of their role
in degrading cellular waste. The lysosome is now recognized as a highly dynamic organelle …

Understanding the basis for cellular growth, proliferation, and function requires determining
the roles of essential genes in diverse cellular processes, including visualizing their …

Image noise is a common problem in light microscopy. This is particularly true in real-time
live-cell imaging applications in which long-term cell viability necessitates low-light …

Scalable sequence–function studies have enabled the systematic analysis and cataloging of
hundreds of thousands of coding and noncoding genetic variants in the human genome …

Optical pooled screening (OPS) is a scalable method for linking image-based phenotypes
with cellular perturbations. However, it has thus far been restricted to relatively low-plex …