[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Parameters necessary to define an immobilized enzyme preparation

J Boudrant, JM Woodley, R Fernandez-Lafuente - Process Biochemistry, 2020 - Elsevier
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …

Enzyme co-immobilization: Always the biocatalyst designers' choice… or not?

S Arana-Peña, D Carballares… - Biotechnology …, 2021 - Elsevier
The increasing relevance of cascade reactions in biocatalysis has sparked a great interest
for enzyme co-immobilization. Enzyme co-immobilization allows access to some kinetic …

Water dynamics in the hydration shells of biomolecules

D Laage, T Elsaesser, JT Hynes - Chemical Reviews, 2017 - ACS Publications
The structure and function of biomolecules are strongly influenced by their hydration shells.
Structural fluctuations and molecular excitations of hydrating water molecules cover a broad …

Water determines the structure and dynamics of proteins

MC Bellissent-Funel, A Hassanali, M Havenith… - Chemical …, 2016 - ACS Publications
Water is an essential participant in the stability, structure, dynamics, and function of proteins
and other biomolecules. Thermodynamically, changes in the aqueous environment affect …

Uniform Ordered Two-Dimensional Mesoporous TiO2 Nanosheets from Hydrothermal-Induced Solvent-Confined Monomicelle Assembly

K Lan, Y Liu, W Zhang, Y Liu, A Elzatahry… - Journal of the …, 2018 - ACS Publications
Two-dimensional (2D) nanomaterials have been the focus of substantial research interest
recently owing to their fascinating and excellent properties. However, 2D porous materials …

Multiscale modeling of aqueous electric double layers

M Becker, P Loche, M Rezaei, A Wolde-Kidan… - Chemical …, 2023 - ACS Publications
From the stability of colloidal suspensions to the charging of electrodes, electric double
layers play a pivotal role in aqueous systems. The interactions between interfaces, water …

Biotechnological relevance of the lipase A from Candida antarctica

RRC Monteiro, JJ Virgen-Ortiz, A Berenguer-Murcia… - Catalysis Today, 2021 - Elsevier
This review intends to present some of the latest studies on the lipase A from Candida
antarctica (CALA). This lipase is among the most stable ones and has some capability to …

[HTML][HTML] Effect of crowding, compartmentalization and nanodomains on protein modification and redox signaling–current state and future challenges

E Fuentes-Lemus, MJ Davies - Free Radical Biology and Medicine, 2023 - Elsevier
Biological milieus are highly crowded and heterogeneous systems where organization of
macromolecules within nanodomains (eg membraneless compartments) is vital to the …

Preparation of a six-enzyme multilayer combi-biocatalyst: Reuse of the most stable enzymes after inactivation of the least stable one

D Carballares, J Rocha-Martin… - ACS Sustainable …, 2022 - ACS Publications
The coimmobilization of six different lipases on octyl agarose beads using an enzyme-
multilayer strategy and employing polyethylenimine (PEI) and glutaraldehyde (GLU) as …