Microfluidic single-cell manipulation and analysis: Methods and applications

T Luo, L Fan, R Zhu, D Sun - Micromachines, 2019 - mdpi.com
In a forest of a hundred thousand trees, no two leaves are alike. Similarly, no two cells in a
genetically identical group are the same. This heterogeneity at the single-cell level has been …

Microfluidic single‐cell omics analysis

X Xu, J Wang, L Wu, J Guo, Y Song, T Tian, W Wang… - Small, 2020 - Wiley Online Library
The commonly existing cellular heterogeneity plays a critical role in biological processes
such as embryonic development, cell differentiation, and disease progress. Single‐cell …

Detection of prostate specific antigen in whole blood by microfluidic chip integrated with dielectrophoretic separation and electrochemical sensing

X Wang, X He, Z He, L Hou, C Ge, L Wang, S Li… - Biosensors and …, 2022 - Elsevier
The efficient detection of cancer markers has faced many challenges, such as severe
interference, complicated and time-consuming operation, low sensitivity and so on. In this …

The revolution of PDMS microfluidics in cellular biology

S Banik, A Uchil, T Kalsang, S Chakrabarty… - Critical Reviews in …, 2023 - Taylor & Francis
Microfluidics is revolutionizing the way research on cellular biology has been traditionally
conducted. The ability to control the cell physicochemical environment by adjusting flow …

High-throughput separation, trapping, and manipulation of single cells and particles by combined dielectrophoresis at a bipolar electrode array

Y Wu, Y Ren, Y Tao, L Hou, H Jiang - Analytical chemistry, 2018 - ACS Publications
Microfluidic systems have been developed widely in scaled-down processes of laboratory
techniques, but they are usually limited in achieving stand-alone functionalities. It is highly …

Microfluidic single-cell transcriptomics: moving towards multimodal and spatiotemporal omics

S Lin, Y Liu, M Zhang, X Xu, Y Chen, H Zhang, C Yang - Lab on a Chip, 2021 - pubs.rsc.org
Cells are the basic units of life with vast heterogeneity. Single-cell transcriptomics unveils
cell-to-cell gene expression variabilities, discovers novel cell types, and uncovers the critical …

Recent progress of microfluidic technology for pharmaceutical analysis

X Jia, X Yang, G Luo, Q Liang - Journal of Pharmaceutical and Biomedical …, 2022 - Elsevier
In recent years, the progress of microfluidic technology has provided new tools for
pharmaceutical analysis and the proposal of pharm-lab-on-a-chip is appealing for its great …

[HTML][HTML] Single-cell assay on microfluidic devices

Q Huang, S Mao, M Khan, JM Lin - Analyst, 2019 - pubs.rsc.org
Advances in microfluidic techniques have prompted researchers to study the inherent
heterogeneity of single cells in cell populations. This would be helpful in the identification of …

Microfluidic device embedding electrodes for dielectrophoretic manipulation of cells‐A review

J Yao, G Zhu, T Zhao, M Takei - Electrophoresis, 2019 - Wiley Online Library
Microfluidic device embedding electrodes realizes cell manipulation with the help of
dielectrophoresis. Cell manipulation is an important technology for cell sorting and cell …

Microfluidics coupled mass spectrometry for single cell multi‐omics

D Zhang, L Qiao - Small Methods, 2024 - Wiley Online Library
Population‐level analysis masks significant heterogeneity between individual cells, making
it difficult to accurately reflect the true intricacies of life activities. Microfluidics is a technique …