Transport of newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi
complex is highly selective. As a general rule, such transport is limited to soluble and …

Eukaryotic cells contain a number of different membrane compartments that have
specialized roles. Each compartment depends on a specific mixture of proteins for its identity …

To dissect secretory traffic, we developed the retention using selective hooks (RUSH)
system. RUSH is a two-state assay based on the reversible interaction of a hook protein …

Nuclear protein HMGB1 is secreted in response to various stimuli and functions as a danger-
associated molecular pattern. Extracellular HMGB1 induces inflammation, cytokine …

Cancer cells exhibit heightened secretory states that drive tumor progression. Here, we
identified a chromosome 3q amplicon that serves as a platform for secretory regulation in …

The Golgi apparatus is a central intracellular membrane-bound organelle with key functions
in trafficking, processing, and sorting of newly synthesized membrane and secretory proteins …

The modification of nuclear, mitochondrial, and cytosolic proteins by O-linked βN-
acetylglucosamine (O-GlcNAc) has emerged as a dynamic and essential post-translational …

A number of long coiled-coil proteins are present on the Golgi. Often referred to as “golgins,”
they are well conserved in evolution and at least five are likely to have been present in the …

The Golgi receives the entire output of newly synthesized cargo from the endoplasmic
reticulum, processes it in the stack largely through modification of bound oligosaccharides …

In vitro studies have suggested that Golgi stack formation involves two homologous
peripheral Golgi proteins, GRASP65 and GRASP55, which localize to the cis and medial …