Synthetic oligonucleotide analogs have greatly aided our understanding of several
biochemical processes. Efficient solid-phase and enzyme-assisted synthetic methods and …

▪ Abstract Just as Darwinian evolution in nature has led to the development of many
sophisticated enzymes, Darwinian evolution in vitro has proven to be a powerful approach …

Background: Several types of RNA enzymes (ribozymes) have been identified in biological
systems and generated in the laboratory. Considering the variety of known RNA enzymes …

Background: Previously we demonstrated that DNA can act as an enzyme in the Pb 2t-
dependent cleavage of an RNA phosphoester. This is a facile reaction, with an uncatalyzed …

Biological catalysis is dominated by enzymes that are made of protein, but several distinct
classes of catalytic RNAs are known to promote chemical transformations that are …

In cells, DNA typically consists of two antiparallel strands arranged in a double-helical
structure, which is central to its fundamental role in storing and transmitting genetic …

The influence of chemical modifications on the catalytic activity and stability of a
hammerhead ribozyme directed against the long terminal repeat RNA of the human …

A systematic study of selectively modified, 36-mer hammerhead ribozymes has resulted in
the identification of a generic, catalytically active and nuclease stable ribozyme motif …

Background: Ribozymes are biological catalysts that promote the hydrolysis and
transesterification of phosphate diesters of RNA. They typically require divalent magnesium …

The hammerhead ribozyme is a small RNA motif that self cleaves at a specific
phosphodiester bond to produce 2′, 3′ cyclic phosphate and 5′ hydroxyl termini …