V-ATPase is the dominant proton pump in plant cells. It contributes to cytosolic pH
homeostasis and energizes transport processes across endomembranes of the secretory …

Over the past decade, technical and methodological improvements in cryogenic electron
microscopy (cryo-EM) single-particle analysis have enabled routine high-resolution …

Limitations to successful single-particle cryo-electron microscopy (cryo-EM) projects include
stable sample generation, production of quality cryo-EM grids with randomly oriented …

Sample thickness is a known key parameter in cryo-electron microscopy (cryo-EM) and can
affect the amount of high-resolution information retained in the image. Yet, common data …

Advances in electron detection have been essential to the success of high-resolution cryo-
EM structure determination. A new generation of direct electron detector called the Apollo …

The increasing popularity and adoption rate of cryo-electron microscopy (cryo-EM) is
evidenced by a growing number of new microscope installations around the world. The …

Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful
technique for the structural characterisation of membrane proteins, especially for targets …

Fusicoccadiene synthase from the fungus Phomopsis amygdali (PaFS) is an assembly-line
terpene synthase that catalyzes the first two steps in the biosynthesis of Fusiccocin A, a …

Single-particle analysis (SPA) by cryo-electron microscopy (cryo-EM) is now a mainstream
technique for high-resolution structural biology. Structure determination by SPA relies upon …

A major goal of biological imaging is localization of biomolecules inside a cell. Fluorescence
microscopy can localize biomolecules inside whole cells and tissues, but its ability to count …