Analysis of intracellular metabolites from microorganisms: quenching and extraction protocols
FR Pinu, SG Villas-Boas, R Aggio - Metabolites, 2017 - mdpi.com
Sample preparation is one of the most important steps in metabolome analysis. The
challenges of determining microbial metabolome have been well discussed within the …
challenges of determining microbial metabolome have been well discussed within the …
The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica
TM Wasylenko, WS Ahn, G Stephanopoulos - Metabolic engineering, 2015 - Elsevier
Oleaginous microbes represent an attractive means of converting a diverse range of
feedstocks into oils that can be transesterified to biodiesel. However, the mechanism of lipid …
feedstocks into oils that can be transesterified to biodiesel. However, the mechanism of lipid …
Current metabolomics: practical applications
The field of metabolomics continues to grow rapidly over the last decade and has been
proven to be a powerful technology in predicting and explaining complex phenotypes in …
proven to be a powerful technology in predicting and explaining complex phenotypes in …
Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms
N Paczia, A Nilgen, T Lehmann, J Gätgens… - Microbial cell …, 2012 - Springer
Overflow metabolism is well known for yeast, bacteria and mammalian cells. It typically
occurs under glucose excess conditions and is characterized by excretions of by-products …
occurs under glucose excess conditions and is characterized by excretions of by-products …
Quantitative evaluation of intracellular metabolite extraction techniques for yeast metabolomics
AB Canelas, A ten Pierick, C Ras, RM Seifar… - Analytical …, 2009 - ACS Publications
Accurate determination of intracellular metabolite levels requires well-validated procedures
for sampling and sample treatment. Several methods exist for metabolite extraction, but the …
for sampling and sample treatment. Several methods exist for metabolite extraction, but the …
Advances in mass spectrometry-based multi-scale metabolomic methodologies and their applications in biological and clinical investigations
Z Wang, H Zhu, W Xiong - Science Bulletin, 2023 - Elsevier
Metabolomics is a nascent field of inquiry that emerged in the late 20 th century. It
encompasses the comprehensive profiling of metabolites across a spectrum of organisms …
encompasses the comprehensive profiling of metabolites across a spectrum of organisms …
The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae
M Ask, M Bettiga, V Mapelli, L Olsson - Biotechnology for biofuels, 2013 - Springer
Background Pretreatment of biomass for lignocellulosic ethanol production generates
compounds that can inhibit microbial metabolism. The furan aldehydes …
compounds that can inhibit microbial metabolism. The furan aldehydes …
Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces …
T Hasunuma, T Sanda, R Yamada, K Yoshimura… - Microbial cell …, 2011 - Springer
Background The development of novel yeast strains with increased tolerance toward
inhibitors in lignocellulosic hydrolysates is highly desirable for the production of bio-ethanol …
inhibitors in lignocellulosic hydrolysates is highly desirable for the production of bio-ethanol …
Application of metabolomics in fungal research
G Li, T Jian, X Liu, Q Lv, G Zhang, J Ling - Molecules, 2022 - mdpi.com
Metabolomics is an essential method to study the dynamic changes of metabolic networks
and products using modern analytical techniques, as well as reveal the life phenomena and …
and products using modern analytical techniques, as well as reveal the life phenomena and …