The quantification of differences between two or more physiological states of a biological
system is among the most important but also most challenging technical tasks in proteomics …

Imaging mass spectrometry combines the chemical specificity and parallel detection of mass
spectrometry with microscopic imaging capabilities. The ability to simultaneously obtain …

A novel MS/MS-based analysis strategy using isotopomer labels, referred to as “tandem
mass tags”(TMTs), for the accurate quantification of peptides and proteins is described. The …

We describe a largely unbiased method for rapid and large-scale proteome analysis by
multidimensional liquid chromatography, tandem mass spectrometry, and database …

A novel, MS‐based approach for the relative quantification of proteins, relying on the
derivatization of primary amino groups in intact proteins using isobaric tag for relative and …

Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …

Proteomics can be viewed as an experimental approach to explain the information
contained in genomic sequences in terms of the structure, function, and control of biological …

Multilevel proteomics aims to delineate proteins at the peptide (bottom‐up proteomics),
proteoform (top‐down proteomics), and protein complex (native proteomics) levels. Capillary …

Proteomics can be defined as the systematic analysis of proteins for their identity, quantity
and function. In contrast to a cell's static genome, the proteome is both complex and …

Central tendency, linear regression, locally weighted regression, and quantile techniques
were investigated for normalization of peptide abundance measurements obtained from …