Opportunities and challenges in long-read sequencing data analysis

SL Amarasinghe, S Su, X Dong, L Zappia, ME Ritchie… - Genome biology, 2020 - Springer
Long-read technologies are overcoming early limitations in accuracy and throughput,
broadening their application domains in genomics. Dedicated analysis tools that take into …

[HTML][HTML] Single-cell and long-read sequencing to enhance modelling of splicing and cell-fate determination

S Wu, U Schmitz - Computational and Structural Biotechnology Journal, 2023 - Elsevier
Single-cell sequencing technologies have revolutionised the life sciences and biomedical
research. Single-cell sequencing provides high-resolution data on cell heterogeneity …

Illuminating the dark side of the human transcriptome with long read transcript sequencing

RI Kuo, Y Cheng, R Zhang, JWS Brown, J Smith… - BMC genomics, 2020 - Springer
Background The human transcriptome annotation is regarded as one of the most complete
of any eukaryotic species. However, limitations in sequencing technologies have biased the …

Error correction enables use of Oxford Nanopore technology for reference-free transcriptome analysis

K Sahlin, P Medvedev - Nature communications, 2021 - nature.com
Oxford Nanopore (ONT) is a leading long-read technology which has been revolutionizing
transcriptome analysis through its capacity to sequence the majority of transcripts from end …

A technology-agnostic long-read analysis pipeline for transcriptome discovery and quantification

D Wyman, G Balderrama-Gutierrez, F Reese, S Jiang… - Biorxiv, 2019 - biorxiv.org
Alternative splicing is widely acknowledged to be a crucial regulator of gene expression and
is a key contributor to both normal developmental processes and disease states. While cost …

tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

L de la Fuente, Á Arzalluz-Luque, M Tardáguila… - Genome Biology, 2020 - Springer
Recent advances in long-read sequencing solve inaccuracies in alternative transcript
identification of full-length transcripts in short-read RNA-Seq data, which encourages the …

De novo clustering of long-read transcriptome data using a greedy, quality value-based algorithm

K Sahlin, P Medvedev - Journal of Computational Biology, 2020 - liebertpub.com
Long-read sequencing of transcripts with Pacific Biosciences (PacBio) Iso-Seq and Oxford
Nanopore Technologies has proven to be central to the study of complex isoform …

[图书][B] Triatominae-The biology of chagas disease vectors

A Guarneri, M Lorenzo - 2021 - Springer
Modern systems biology has much to owe to the concept of “model species.” This label
refers to organisms that are privileged for the study of particular biological phenomena …

Accurate spliced alignment of long RNA sequencing reads

K Sahlin, V Mäkinen - Bioinformatics, 2021 - academic.oup.com
Motivation Long-read RNA sequencing technologies are establishing themselves as the
primary techniques to detect novel isoforms, and many such analyses are dependent on …

Amplicon_sorter: A tool for reference‐free amplicon sorting based on sequence similarity and for building consensus sequences

AR Vierstraete, BP Braeckman - Ecology and Evolution, 2022 - Wiley Online Library
Abstract Oxford Nanopore Technologies (ONT) is a third‐generation sequencing technology
that is gaining popularity in ecological research for its portable and low‐cost sequencing …