Cancer 'genetic dependencies'—genes whose products are essential for cancer cell fitness—
are promising targets for therapeutic development. However, recent evidence has cast doubt …

Functional genomics describes a field of biology that uses a range of approaches for
assessing gene function with high-throughput molecular, genetic, and cellular technologies …

During CRISPR-directed gene editing, multiple gene repair mechanisms interact to produce
a wide and largely unpredictable variety of sequence changes across an edited population …

The alternative non-homologous end-joining (NHEJ) pathway promotes DNA double-strand
break (DSB) repair in cells deficient for NHEJ or homologous recombination, suggesting that …

Simple Summary The synthetic lethality (SL) clinical success of PARP inhibitors in
homologous recombinant deficient tumors has established a new concept for cancer …

Homology-directed repair (HDR) of a DNA break allows copying of genetic material from an
exogenous DNA template and is frequently exploited in CRISPR-Cas9 genome editing …

The use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 has
moved from bench to bedside in less than 10years, realising the vision of correcting disease …

The use of CRISPR/Cas endonucleases has revolutionized gene editing techniques for
research on Chlamydomonas reinhardtii. To better utilize the CRISPR/Cas system, it is …

Pathogenic variants in RAD51C confer an elevated risk of breast and ovarian cancer, while
individuals homozygous for specific RAD51C alleles may develop Fanconi anemia. Using …

DNA polymerase theta (Polθ)-mediated end joining (TMEJ) is, along with homologous
recombination (HR) and non-homologous end-joining (NHEJ), one of the most important …