Highlights•The accessibility to automated flow cytometry equipment has recently been
increased through the open science initiative.•Automated and reactive flow cytometry can be …

The ability of bacteria to respond to changes in their environment is critical to their survival,
allowing them to withstand stress, form complex communities, and induce virulence …

Bacterial populations are highly adaptive. They can respond to stress and survive in shifting
environments. How the behaviours of individual bacteria vary during stress, however, is …

Genetically identical bacterial cells commonly display different phenotypes. This phenotypic
heterogeneity is well known for stress responses, where it is often explained as bet hedging …

Highlights•Bacteria have common and species-specific mechanisms to survive acid
stress.•Acid stress response and antibiotic tolerance are interconnected.•Acid stress …

Highlights•Heterogeneity in phenotype and growth rate of single cells affect stress
survival.•Single-cell stress responses in biofilms and interspecies interactions can alter …

Isogenic cell populations can cope with stress conditions by switching to alternative
phenotypes. Even if it can lead to increased fitness in a natural context, this feature is …

Bacteria employ diverse gene regulatory networks to survive stress, but deciphering the
underlying logic of these complex networks has proved challenging. Here, we use time …

Gene expression is inherently dynamic, due to complex regulation and stochastic
biochemical events. However, the effects of these dynamics on cell phenotypes can be …

Antibiotics typically induce major physiological changes in bacteria. However, their effect on
nutrient consumption remains unclear. Here we found that Escherichia coli communities can …