Understanding plant pathogen interactions using spatial and single-cell technologies

J Zhu, A Moreno-Pérez, G Coaker - Communications Biology, 2023 - nature.com
Plants are in contact with diverse pathogens and microorganisms. Intense investigation over
the last 30 years has resulted in the identification of multiple immune receptors in model and …

Scalable single-cell profiling of chromatin modifications with sciCUT&Tag

DH Janssens, JE Greene, SJ Wu, CA Codomo… - Nature …, 2024 - nature.com
Cleavage under targets and tagmentation (CUT&Tag) is an antibody-directed in situ
chromatin profiling strategy that is rapidly replacing immune precipitation-based methods …

[HTML][HTML] A single-cell time-lapse of mouse prenatal development from gastrula to birth

C Qiu, BK Martin, IC Welsh, RM Daza, TM Le, X Huang… - Nature, 2024 - nature.com
The house mouse (Mus musculus) is an exceptional model system, combining genetic
tractability with close evolutionary affinity to humans,. Mouse gestation lasts only 3 weeks …

Chromatin context-dependent regulation and epigenetic manipulation of prime editing

X Li, W Chen, BK Martin, D Calderon, C Lee, J Choi… - Cell, 2024 - cell.com
We set out to exhaustively characterize the impact of the cis-chromatin environment on
prime editing, a precise genome engineering tool. Using a highly sensitive method for …

A global view of aging and Alzheimer's pathogenesis-associated cell population dynamics and molecular signatures in human and mouse brains

A Sziraki, Z Lu, J Lee, G Banyai, S Anderson… - Nature Genetics, 2023 - nature.com
Conventional methods fall short in unraveling the dynamics of rare cell types related to
aging and diseases. Here we introduce EasySci, an advanced single-cell combinatorial …

Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters

JB Lalanne, SG Regalado, S Domcke, D Calderon… - Nature …, 2024 - nature.com
The inability to scalably and precisely measure the activity of developmental cis-regulatory
elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a …

Retinoic acid induces human gastruloids with posterior embryo-like structures

N Hamazaki, W Yang, CA Kubo, C Qiu, BK Martin… - Nature Cell …, 2024 - nature.com
Gastruloids are a powerful in vitro model of early human development. However, although
elongated and composed of all three germ layers, human gastruloids do not morphologically …

Four Core Genotypes mice harbour a 3.2MB XY translocation that perturbs Tlr7 dosage

J Panten, S Del Prete, JP Cleland, LM Saunders… - Nature …, 2024 - nature.com
Abstract The Four Core Genotypes (FCG) is a mouse model system used to disentangle the
function of sex chromosomes and hormones. We report that a copy of a 3.2 MB region of the …

Recent Progress in MOF‐Aerogel Fabrication and Applications

T Guo, H Mashhadimoslem, L Choopani, MM Salehi… - Small, 2024 - Wiley Online Library
Recent advancements in metal–organic frameworks (MOFs) underscore their significant
potential in chemical and materials research, owing to their remarkable properties and …

Transcriptomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomy

H Li, D Li, N Ledru, Q Xuanyuan, H Wu, A Asthana… - Cell metabolism, 2024 - cell.com
A large-scale multimodal atlas that includes major kidney regions is lacking. Here, we
employed simultaneous high-throughput single-cell ATAC/RNA sequencing (SHARE-seq) …