Dicer-independent processing of small RNA duplexes: mechanistic insights and applications
E Herrera-Carrillo, B Berkhout - Nucleic acids research, 2017 - academic.oup.com
MicroRNAs (miRNAs) play a pivotal role in the regulation of cellular gene expression via the
conserved RNA interference (RNAi) mechanism. Biogenesis of the unusual miR-451 does …
conserved RNA interference (RNAi) mechanism. Biogenesis of the unusual miR-451 does …
CRISPR therapy towards an HIV cure
E Herrera-Carrillo, Z Gao… - Briefings in functional …, 2020 - academic.oup.com
Tools based on RNA interference (RNAi) and the recently developed clustered regularly
short palindromic repeats (CRISPR) system enable the selective modification of gene …
short palindromic repeats (CRISPR) system enable the selective modification of gene …
Delineation of the exact transcription termination signal for type 3 polymerase III
Z Gao, E Herrera-Carrillo, B Berkhout - Molecular Therapy-Nucleic Acids, 2018 - cell.com
Type 3 Pol III promoters such as U6 are widely used for expression of small RNAs, including
short hairpin RNA for RNAi applications and guide RNA in CRISPR genome-editing …
short hairpin RNA for RNAi applications and guide RNA in CRISPR genome-editing …
Short hairpin RNAs for strand-specific small interfering RNA production
RNA interference (RNAi) is an effective mechanism for inhibiting gene expression at the post-
transcriptional level. Expression of a messenger RNA (mRNA) can be inhibited by a∼ 22 …
transcriptional level. Expression of a messenger RNA (mRNA) can be inhibited by a∼ 22 …
Mutation of nucleotides around the+ 1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage
ABSTRACT Type 3 RNA polymerase III (Pol III) promoters are widely used for the expression
of small RNAs such as short hairpin RNA and guide RNA in the popular RNAi and CRISPR …
of small RNAs such as short hairpin RNA and guide RNA in the popular RNAi and CRISPR …
RNA polymerase II activity of type 3 Pol III promoters
Z Gao, E Herrera-Carrillo, B Berkhout - Molecular Therapy-Nucleic Acids, 2018 - cell.com
In eukaryotes, three RNA polymerases (Pol I, II, and III) are responsible for the transcription
of distinct subsets of genes. Gene-external type 3 Pol III promoters use defined transcription …
of distinct subsets of genes. Gene-external type 3 Pol III promoters use defined transcription …
VEGFA-targeting miR-agshRNAs combine efficacy with specificity and safety for retinal gene therapy
Retinal gene therapy using RNA interference (RNAi) to silence targeted genes requires both
efficacy and safety. Short hairpin RNAs (shRNAs) are useful for RNAi, but high expression …
efficacy and safety. Short hairpin RNAs (shRNAs) are useful for RNAi, but high expression …
Recent developments in targeting genes and pathways by RNAi‐based approaches in colorectal cancer
A Jebelli, B Baradaran, J Mosafer… - Medicinal Research …, 2021 - Wiley Online Library
A wide spectrum of genetic and epigenetic variations together with environmental factors
has made colorectal cancer (CRC), which involves the colon and rectum, a challenging and …
has made colorectal cancer (CRC), which involves the colon and rectum, a challenging and …
[HTML][HTML] RIG-I recognition of RNA targets: the influence of terminal base pair sequence and overhangs on affinity and signaling
Within the complex environment of the human cell, the RIG-I innate immune receptor must
detect the presence of double-stranded viral RNA molecules and differentiate them from a …
detect the presence of double-stranded viral RNA molecules and differentiate them from a …
Efficient knockdown and lack of passenger strand activity by Dicer-independent shRNAs expressed from Pol II-driven microRNA scaffolds
E Kaadt, S Alsing, CR Cecchi, CK Damgaard… - … Therapy-Nucleic Acids, 2019 - cell.com
The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-
processed passenger strand. Recent studies have shown that shortening the stem of …
processed passenger strand. Recent studies have shown that shortening the stem of …