Metabolic engineering: methodologies and applications

MJ Volk, VG Tran, SI Tan, S Mishra, Z Fatma… - Chemical …, 2022 - ACS Publications
Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Microbial production of advanced biofuels

J Keasling, H Garcia Martin, TS Lee… - Nature Reviews …, 2021 - nature.com
Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …

Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Metabolic engineering of Escherichia coli for natural product biosynthesis

D Yang, SY Park, YS Park, H Eun, SY Lee - Trends in Biotechnology, 2020 - cell.com
Natural products are widely employed in our daily lives as food additives, pharmaceuticals,
nutraceuticals, and cosmetic ingredients, among others. However, their supply has often …

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

K Nishida, T Arazoe, N Yachie, S Banno, M Kakimoto… - Science, 2016 - science.org
INTRODUCTION To combat invading pathogens, cells develop an adaptive immune
response by changing their own genetic information. In vertebrates, the generation of …

Synthetic biology strategies for microbial biosynthesis of plant natural products

A Cravens, J Payne, CD Smolke - Nature communications, 2019 - nature.com
Metabolic engineers endeavor to create a bio-based manufacturing industry using microbes
to produce fuels, chemicals, and medicines. Plant natural products (PNPs) are historically …

Synthetic protein condensates for cellular and metabolic engineering

ZG Qian, SC Huang, XX Xia - Nature chemical biology, 2022 - nature.com
Protein condensates are distinct structures assembled in living cells that concentrate
molecules via phase separation in a confined subcellular compartment. In the past decade …

Endothelial cell metabolism

G Eelen, P de Zeeuw, L Treps, U Harjes… - Physiological …, 2018 - journals.physiology.org
Endothelial cells (ECs) are more than inert blood vessel lining material. Instead, they are
active players in the formation of new blood vessels (angiogenesis) both in health and (life …

Multienzymatic cascade reactions via enzyme complex by immobilization

ET Hwang, S Lee - ACS Catalysis, 2019 - ACS Publications
Multienzymatic cascade reactions are a most important technology to succeed in industrial
process development, such as synthesis of pharmaceutical, cosmetic, and nutritional …

Engineering cellular metabolism

J Nielsen, JD Keasling - Cell, 2016 - cell.com
Metabolic engineering is the science of rewiring the metabolism of cells to enhance
production of native metabolites or to endow cells with the ability to produce new products …