In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Radio signals from live cells: the coming of age of in-cell solution NMR

E Luchinat, M Cremonini, L Banci - Chemical reviews, 2022 - ACS Publications
A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

NMR-based methods for protein analysis

Y Hu, K Cheng, L He, X Zhang, B Jiang… - Analytical …, 2021 - ACS Publications
Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch

A Bah, RM Vernon, Z Siddiqui, M Krzeminski… - Nature, 2015 - nature.com
Intrinsically disordered proteins play important roles in cell signalling, transcription,
translation and cell cycle regulation,. Although they lack stable tertiary structure, many …

Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs)

FX Theillet, A Binolfi, T Frembgen-Kesner… - Chemical …, 2014 - ACS Publications
It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …

How phosphorylation impacts intrinsically disordered proteins and their function

EA Newcombe, E Delaforge… - Essays in …, 2022 - portlandpress.com
Phosphorylation is the most common post-translational modification (PTM) in eukaryotes,
occurring particularly frequently in intrinsically disordered proteins (IDPs). These proteins …

Exploring free-energy landscapes of intrinsically disordered proteins at atomic resolution using NMR spectroscopy

MR Jensen, M Zweckstetter, J Huang… - Chemical …, 2014 - ACS Publications
The last 15 years have seen a paradigm shift in our understanding of protein biochemistry,
with the realization that an unexpectedly high fraction of the human genome codes for …

[HTML][HTML] Choosing membrane mimetics for NMR structural studies of transmembrane proteins

DE Warschawski, AA Arnold, M Beaugrand… - … et Biophysica Acta (BBA …, 2011 - Elsevier
The native environment of membrane proteins is complex and scientists have felt the need
to simplify it to reduce the number of varying parameters. However, experimental problems …

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

K Inomata, A Ohno, H Tochio, S Isogai, T Tenno… - Nature, 2009 - nature.com
In-cell NMR is an isotope-aided multi-dimensional NMR technique that enables
observations of conformations and functions of proteins in living cells at the atomic level …

In-cell NMR: Why and how?

FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …