In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

NMR-based methods for protein analysis

Y Hu, K Cheng, L He, X Zhang, B Jiang… - Analytical …, 2021 - ACS Publications
Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

Advanced techniques for detecting protein misfolding and aggregation in cellular environments

Y Bai, S Zhang, H Dong, Y Liu, C Liu… - Chemical Reviews, 2023 - ACS Publications
Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …

A natural product inhibits the initiation of α-synuclein aggregation and suppresses its toxicity

M Perni, C Galvagnion, A Maltsev… - Proceedings of the …, 2017 - National Acad Sciences
The self-assembly of α-synuclein is closely associated with Parkinson's disease and related
syndromes. We show that squalamine, a natural product with known anticancer and antiviral …

Understanding protein non-folding

VN Uversky, AK Dunker - Biochimica et Biophysica Acta (BBA)-Proteins …, 2010 - Elsevier
This review describes the family of intrinsically disordered proteins, members of which fail to
form rigid 3-D structures under physiological conditions, either along their entire lengths or …

In-cell NMR: Why and how?

FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Weak chemical interactions that drive protein evolution: crowding, sticking, and quinary structure in folding and function

D Guin, M Gruebele - Chemical reviews, 2019 - ACS Publications
In recent years, better instrumentation and greater computing power have enabled the
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …

Protein structure determination in living cells by in-cell NMR spectroscopy

D Sakakibara, A Sasaki, T Ikeya, J Hamatsu… - Nature, 2009 - nature.com
Investigating proteins 'at work'in a living environment at atomic resolution is a major goal of
molecular biology, which has not been achieved even though methods for the three …

Observing biological dynamics at atomic resolution using NMR

AK Mittermaier, LE Kay - Trends in biochemical sciences, 2009 - cell.com
Biological macromolecules are highly flexible and continually undergo conformational
fluctuations on a broad spectrum of timescales. It has long been recognized that dynamics …

Protein dynamics and function from solution state NMR spectroscopy

M Kovermann, P Rogne, M Wolf-Watz - Quarterly reviews of …, 2016 - cambridge.org
It is well-established that dynamics are central to protein function; their importance is
implicitly acknowledged in the principles of the Monod, Wyman and Changeux model of …