Rubisco function, evolution, and engineering

N Prywes, NR Phillips, OT Tuck… - Annual review of …, 2023 - annualreviews.org
Carbon fixation is the process by which CO2 is converted from a gas into biomass. The
Calvin–Benson–Bassham cycle (CBB) is the dominant carbon-consuming pathway on …

Macromolecular crowding, phase separation, and homeostasis in the orchestration of bacterial cellular functions

B Monterroso, W Margolin, AJ Boersma, G Rivas… - Chemical …, 2024 - ACS Publications
Macromolecular crowding affects the activity of proteins and functional macromolecular
complexes in all cells, including bacteria. Crowding, together with physicochemical …

The eukaryotic linear motif resource: 2022 release

M Kumar, S Michael, J Alvarado-Valverde… - Nucleic acids …, 2022 - academic.oup.com
Almost twenty years after its initial release, the Eukaryotic Linear Motif (ELM) resource
remains an invaluable source of information for the study of motif-mediated protein-protein …

Modelling the pyrenoid-based CO2-concentrating mechanism provides insights into its operating principles and a roadmap for its engineering into crops

C Fei, AT Wilson, NM Mangan, NS Wingreen… - Nature Plants, 2022 - nature.com
Many eukaryotic photosynthetic organisms enhance their carbon uptake by supplying
concentrated CO2 to the CO2-fixing enzyme Rubisco in an organelle called the pyrenoid …

Identification of a carbonic anhydrase–Rubisco complex within the alpha-carboxysome

C Blikstad, EJ Dugan, TG Laughlin… - Proceedings of the …, 2023 - National Acad Sciences
Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO2 fixation—
Rubisco and carbonic anhydrase—and are the centerpiece of the bacterial CO2 …

New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields

L Adler, A Díaz-Ramos, Y Mao, KR Pukacz… - Plant …, 2022 - academic.oup.com
Many photosynthetic species have evolved CO2-concentrating mechanisms (CCMs) to
improve the efficiency of CO2 assimilation by Rubisco and reduce the negative impacts of …

Deep learning improves macromolecule identification in 3D cellular cryo-electron tomograms

E Moebel, A Martinez-Sanchez, L Lamm, RD Righetto… - Nature …, 2021 - nature.com
Cryogenic electron tomography (cryo-ET) visualizes the 3D spatial distribution of
macromolecules at nanometer resolution inside native cells. However, automated …

A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage

J Barrett, MIS Naduthodi, Y Mao, C Dégut, S Musiał… - Nature Plants, 2024 - nature.com
CO2 fixation is commonly limited by inefficiency of the CO2-fixing enzyme Rubisco.
Eukaryotic algae concentrate and fix CO2 in phase-separated condensates called …

[HTML][HTML] Pyrenoids: CO2-fixing phase separated liquid organelles

J Barrett, P Girr, LCM Mackinder - … et Biophysica Acta (BBA)-Molecular Cell …, 2021 - Elsevier
Pyrenoids are non-membrane bound organelles found in chloroplasts of algae and hornwort
plants that can be seen by light-microscopy. Pyrenoids are formed by liquid-liquid phase …

[HTML][HTML] Phase separation in biology and disease; current perspectives and open questions

S Boeynaems, S Chong, J Gsponer, L Holt… - Journal of molecular …, 2023 - Elsevier
In the past almost 15 years, we witnessed the birth of a new scientific field focused on the
existence, formation, biological functions, and disease associations of membraneless …