Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection,
quantification, and typing of different microbial agents in the areas of clinical and veterinary …

While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

Digital polymerase chain reaction (dPCR) technology has provided a new technique for
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …

The clinical management of cancer has evolved in recent years towards more personalized
strategies that require a comprehensive knowledge of the complex molecular features …

Over the past decade numerous publications have demonstrated how digital PCR (dPCR)
enables precise and sensitive quantification of nucleic acids in a wide range of applications …

The number of genetically modified organisms (GMOs) on the market is steadily increasing.
Because of regulation of cultivation and trade of GMOs in several countries, there is …

High‐throughput identification technologies provide efficient tools for understanding the
ecology and functioning of microorganisms. Yet, these methods have been only rarely used …

One of the main challenges in the gene therapy viral vector development is to establish an
optimized process for its large scale production. This requires optimization for upstream and …

Droplet digital polymerase chain reaction (ddPCR) is a novel molecular biology technique
providing absolute quantification of target nucleic acids without the need for an external …