The introduction of potent combination therapies in the mid-90s had a tremendous effect on
AIDS mortality. However, drug resistance has been a major factor contributing to …

Human immunodeficiency virus (HIV) causes approximately 2.5 million new infections every
year, and nearly 1.6 million patients succumb to HIV each year. Several factors, including …

Background Human immunodeficiency virus type 1 (HIV-1) has a biased nucleotide
composition different from human genes. This raises the question of how evolution has …

In retroviruses, strand displacement DNA-dependent DNA polymerization catalyzed by the
viral reverse transcriptase (RT) is required to synthesize double-stranded proviral DNA. In …

Coupled with PCR, reverse transcriptases (RTs) have been widely used for RNA detection
and gene expression analysis. Increased thermostability and nucleic acid binding affinity are …

Abstract HIV-1 reverse transcriptase (RT) connection subdomain mutations at positions 348,
369 and 376 have been associated with resistance to non-nucleoside RT inhibitors …

We analyzed a multi-drug resistant (MR) HIV-1 reverse transcriptase (RT), subcloned from a
patient-derived subtype CRF02_AG, harboring 45 amino acid exchanges, amongst them …

Synonymous genome recoding has been widely used to study different aspects of virus
biology. Codon usage affects the temporal regulation of viral gene expression. In this study …

Hepatitis B virus (HBV) is a hepatotropic, partially double-stranded DNA virus that replicates
by reverse transcription and is a major cause of chronic liver disease and hepatocellular …

We evaluated fidelity of various reverse transcriptases (RTs) by a novel method with
modified next-generation sequencing (NGS). In the optimized condition, one NGS run could …