Biological research has focused in the past on model organisms and most of the functional
genomics studies in the field of plant sciences are still performed on model species or …

During the last decade, protein analysis and proteomics have been established as new tools
for understanding various biological problems. As the identification of proteins after classical …

Autophagy is a dynamic membrane phenomenon for bulk protein degradation in the
lysosome/vacuole,. Apg8/Aut7 is an essential factor for autophagy in yeast,,. We previously …

Novel protein discovery and immunopeptidomics depend on highly sensitive de novo
peptide sequencing with tandem mass spectrometry. Despite notable improvement using …

For the identification of novel proteins using MS/MS, de novo sequencing software computes
one or several possible amino acid sequences (called sequence tags) for each MS/MS …

De novo peptide sequencing has improved remarkably in the past decade as a result of
better instruments and computational algorithms. However, de novo sequencing can …

Proteomics is the study of proteins, their time-and location-dependent expression profiles, as
well as their modifications and interactions. Mass spectrometry is useful to investigate many …

The crystal structure of the heterotrimeric quinohemoprotein amine dehydrogenase from
Paracoccus denitrificans has been determined at 2.05-Å resolution. Within an 82-residue …

In hot electron capture dissociation (HECD), multiply protonated polypeptides fragment upon
capturing∼ 11-eV electrons. The excess of energy upon the primary c, z• cleavage induces …

De novo peptide sequencing is the only tool for extracting peptide sequences directly from
tandem mass spectrometry (MS) data without any protein database. However, neither the …