[HTML][HTML] The CRISPR-Cas toolbox and gene editing technologies
G Liu, Q Lin, S Jin, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …
technologies, which are widely used in the life sciences. To improve the performance of …
CRISPR-based therapeutics: current challenges and future applications
The discovery, only a decade ago, of the genome editing power of clustered regularly
interspaced short palindromic repeats (CRISPR)-associated nucleases is already …
interspaced short palindromic repeats (CRISPR)-associated nucleases is already …
Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors
The viral delivery of base editors has been complicated by their size and by the limited
packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches …
packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches …
A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
Y Wang, T Qi, J Liu, Y Yang, Z Wang, Y Wang… - Science …, 2023 - science.org
The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end
joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide …
joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide …
Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility
CRISPR–Cas9 genome engineering is a powerful technology for correcting genetic
diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a …
diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a …
A split and inducible adenine base editor for precise in vivo base editing
DNA base editors use deaminases fused to a programmable DNA-binding protein for
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
Global detection of DNA repair outcomes induced by CRISPR–Cas9
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA
Cas12f is a hypercompact type V, Cas12 family member. Previously, we reported a set of
engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here …
engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here …
Rationally designed base editors for precise editing of the sickle cell disease mutation
Base editors are fusions of a deaminase and CRISPR-Cas ribonucleoprotein that allow
programmable installment of transition mutations without double-strand DNA break …
programmable installment of transition mutations without double-strand DNA break …