Cell culture in microfluidic droplets

S Sart, G Ronteix, S Jain, G Amselem… - Chemical …, 2022 - ACS Publications
Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …

Droplet-based microfluidics in biomedical applications

L Amirifar, M Besanjideh, R Nasiri, A Shamloo… - …, 2022 - iopscience.iop.org
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Intelligent microfluidics: The convergence of machine learning and microfluidics in materials science and biomedicine

EA Galan, H Zhao, X Wang, Q Dai, WTS Huck, S Ma - Matter, 2020 - cell.com
Microfluidics permit the automated manipulation of fluids at the microscale with high
throughput and spatiotemporal precision, enabling the generation of large, multidimensional …

Recent advances in manipulation of micro-and nano-objects with magnetic fields at small scales

Q Cao, Q Fan, Q Chen, C Liu, X Han, L Li - Materials Horizons, 2020 - pubs.rsc.org
Manipulation of micro-and nano-objects has been widely studied in the past two decades
due to its potential applications in various fields. In this review, we focus primarily on …

Droplet microfluidics: from proof-of-concept to real-world utility?

A Suea-Ngam, PD Howes, M Srisa-Art… - Chemical …, 2019 - pubs.rsc.org
Droplet microfluidics constitutes a diverse and practical tool set that enables chemical and
biological experiments to be performed at high speed and with enhanced efficiency when …

High‐precision stereolithography of biomicrofluidic devices

AP Kuo, N Bhattacharjee, YS Lee… - Advanced materials …, 2019 - Wiley Online Library
Stereolithography (SL) is emerging as an attractive alternative to soft lithography for
fabricating microfluidic devices due to its low cost and high design efficiency. Low molecular …

Ultrahigh-throughput screening enables efficient single-round oxidase remodelling

A Debon, M Pott, R Obexer, AP Green, L Friedrich… - Nature Catalysis, 2019 - nature.com
Biocatalysis provides a potentially sustainable means of chemical manufacturing. However,
the tailoring of enzymes to industrial processes is often laborious and time consuming …

Speeding up enzyme discovery and engineering with ultrahigh-throughput methods

HA Bunzel, X Garrabou, M Pott, D Hilvert - Current opinion in structural …, 2018 - Elsevier
Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-
throughput screening methods for rapid analysis of millions of variants are therefore …

Substantial Improvement of an Epimerase for the Synthesis of D‐Allulose by Biosensor‐Based High‐Throughput Microdroplet Screening

C Li, X Gao, H Qi, W Zhang, L Li, C Wei… - Angewandte …, 2023 - Wiley Online Library
Biosynthesis of D‐allulose has been achieved using ketose 3‐epimerases (KEases), but its
application is limited by poor catalytic performance. In this study, we redesigned a …

Intelligent acoustofluidics enabled mini-bioreactors for human brain organoids

H Cai, Z Ao, Z Wu, S Song, K Mackie, F Guo - Lab on a Chip, 2021 - pubs.rsc.org
Acoustofluidics, by combining acoustics and microfluidics, provides a unique means to
manipulate cells and liquids for broad applications in biomedical sciences and translational …