Tools and strategies of systems metabolic engineering for the development of microbial cell factories for chemical production
Sustainable production of chemicals from renewable non-food biomass has become a
promising alternative to overcome environmental issues caused by our heavy dependence …
promising alternative to overcome environmental issues caused by our heavy dependence …
[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
[HTML][HTML] Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
[HTML][HTML] A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing
CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …
[HTML][HTML] In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing
Y Wang, H Cheng, Y Liu, Y Liu, X Wen, K Zhang… - Nature …, 2021 - nature.com
Reprogramming complex cellular metabolism requires simultaneous regulation of multigene
expression. Ex-situ cloning-based methods are commonly used, but the target gene number …
expression. Ex-situ cloning-based methods are commonly used, but the target gene number …
[HTML][HTML] Recent Advances in Silent Gene Cluster Activation in Streptomyces
Z Liu, Y Zhao, C Huang, Y Luo - Frontiers in Bioengineering and …, 2021 - frontiersin.org
Natural products (NPs) are critical sources of drug molecules for decades. About two-thirds
of natural antibiotics are produced by Streptomyces. Streptomyces have a large number of …
of natural antibiotics are produced by Streptomyces. Streptomyces have a large number of …
CRISPR screens in plants: approaches, guidelines, and future prospects
C Gaillochet, W Develtere, TB Jacobs - The Plant Cell, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems
have revolutionized genome engineering by facilitating a wide range of targeted DNA …
have revolutionized genome engineering by facilitating a wide range of targeted DNA …
Microbial chassis engineering drives heterologous production of complex secondary metabolites
The cryptic secondary metabolite biosynthetic gene clusters (BGCs) far outnumber currently
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …
Activating cryptic biosynthetic gene cluster through a CRISPR–Cas12a-mediated direct cloning approach
M Liang, L Liu, F Xu, X Zeng, R Wang… - Nucleic Acids …, 2022 - academic.oup.com
Direct cloning of biosynthetic gene clusters (BGCs) from microbial genomes facilitates
natural product-based drug discovery. Here, by combining Cas12a and the advanced …
natural product-based drug discovery. Here, by combining Cas12a and the advanced …
[HTML][HTML] Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes
Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …