Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …

Technological applications in agriculture have evolved substantially to increase crop yields
and quality to meet global food demand. Conventional techniques, such as seed saving …

Genome engineering has been re-shaping plant biotechnology and agriculture. Crop
improvement using the recently developed gene editing techniques is now easier, faster …

Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has
been widely used for genome editing in mammalian cells and plants due to its distinct …

Key message We have established a DNA-free genome editing method via
ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants …

Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been
widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique …

Genome editing technology encompasses a revolutionary set of molecular tools that holds
the potential to improve human health, unlocking improvements in medicine and agriculture …

CRISPR/Cas9 is a powerful genome editing tool applicable to diverse plant species.
However, the application of CRISPR/Cas9-ribonucleoproteins (RNPs) in oil palm (Elaeis …

Climate change and rapid adaption of invasive pathogens pose a constant pressure on the
fruit industry to develop improved varieties. Aiming to accelerate the development of better …

CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a
technology that research scientists use to selectively modify the DNA of living organisms …