The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Recently, there has been an increase in the demand for enzymes with modified activity,
specificity, and stability. Enzyme engineering is an important tool to meet the demand for …

Improvement in intrinsic enzymatic features is in many instances a prerequisite for the
scalable applicability of many industrially important biocatalysts. To this end, various …

Cell surface engineering is a rapidly developing technology of microorganism that achieves
modification of cell surface function by joining external functional peptides with surface …

Enzyme design and engineering strategies are typically constrained by the limited size of
nature's genetic alphabet, comprised of only 20 canonical amino acids. In recent years, site …

Protein chemical modifications are important tools for elucidating chemical and biological
functions of proteins. Several strategies have been developed to implement these …

In enzyme engineering, the main targets for enhancing properties are enzyme activity,
stereoselective specificity, stability, substrate range, and the development of unique …

Non-canonical amino acids (ncAAs) have been utilized as an invaluable tool for modulating
the active site of the enzymes, probing the complex enzyme mechanisms, improving …

Octyl formate is an important substance used in the perfume industry in products such as
cosmetics, perfumes, and flavoring. Octyl formate is mostly produced by chemical catalysts …

Stabilizing proteins against the thermal stress or proteolytic attacks is an important goal in
many protein engineering studies. As the simplest approach to the rational engineering of …