The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

A detailed landscape of CRISPR-Cas-mediated plant disease and pest management

S Karmakar, P Das, D Panda, K Xie, MJ Baig, KA Molla - Plant Science, 2022 - Elsevier
Genome editing technology has rapidly evolved to knock-out genes, create targeted genetic
variation, install precise insertion/deletion and single nucleotide changes, and perform large …

[HTML][HTML] Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy

X Huang, H Jia, J Xu, Y Wang, J Wen, N Wang - Nature Plants, 2023 - nature.com
Transgene-free plant genome editing in the T0 generation is highly desirable but
challenging,. Here we achieved such a goal using a co-editing strategy via Agrobacterium …

Genome editing for plant research and crop improvement

X Zhan, Y Lu, JK Zhu, JR Botella - Journal of Integrative Plant …, 2021 - Wiley Online Library
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

[HTML][HTML] CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement

A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

[HTML][HTML] Highly efficient generation of canker-resistant sweet orange enabled by an improved CRISPR/Cas9 system

X Huang, Y Wang, N Wang - Frontiers in Plant Science, 2022 - frontiersin.org
Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

[HTML][HTML] The rise of the CRISPR/Cpf1 system for efficient genome editing in plants

A Alok, D Sandhya, P Jogam, V Rodrigues… - Frontiers in plant …, 2020 - frontiersin.org
Cpf1, an endonuclease of the class 2 CRISPR family, fills the gaps that were previously
faced in the world of genome engineering tools, which include the TALEN, ZFN, and …

[HTML][HTML] Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9

J Ji, C Zhang, Z Sun, L Wang, D Duanmu… - International Journal of …, 2019 - mdpi.com
Cowpea (Vigna unguiculata) is widely cultivated across the world. Due to its symbiotic
nitrogen fixation capability and many agronomically important traits, such as tolerance to low …

Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement

Q Li, M Sapkota, E van der Knaap - Horticulture research, 2020 - academic.oup.com
Directed breeding of horticultural crops is essential for increasing yield, nutritional content,
and consumer-valued characteristics such as shape and color of the produce. However …