How introns enhance gene expression

O Shaul - The international journal of biochemistry & cell biology, 2017 - Elsevier
In many eukaryotes, including mammals, plants, yeast, and insects, introns can increase
gene expression without functioning as a binding site for transcription factors. This …

The exon junction complex as a node of post-transcriptional networks

HL Hir, J Saulière, Z Wang - Nature reviews Molecular cell biology, 2016 - nature.com
The exon junction complex (EJC) is deposited onto mRNAs following splicing and adopts a
unique structure, which can both stably bind to mRNAs and function as an anchor for diverse …

RNA ribose methylation (2′-O-methylation): Occurrence, biosynthesis and biological functions

L Ayadi, A Galvanin, F Pichot, V Marchand… - Biochimica et Biophysica …, 2019 - Elsevier
Methylation of riboses at 2′-OH group is one of the most common RNA modifications found
in number of cellular RNAs from almost any species which belong to all three life domains …

[HTML][HTML] The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus

G Singh, A Kucukural, C Cenik, JD Leszyk, SA Shaffer… - Cell, 2012 - cell.com
In addition to sculpting eukaryotic transcripts by removing introns, pre-mRNA splicing greatly
impacts protein composition of the emerging mRNP. The exon junction complex (EJC) …

CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex

J Saulière, V Murigneux, Z Wang… - Nature structural & …, 2012 - nature.com
The exon junction complex (EJC) is a central effector of the fate of mRNAs, linking nuclear
processing to mRNA transport, translation and surveillance. However, little is known about …

Exon junction complex shapes the transcriptome by repressing recursive splicing

L Blazquez, W Emmett, R Faraway, JMB Pineda… - Molecular cell, 2018 - cell.com
Recursive splicing (RS) starts by defining an" RS-exon," which is then spliced to the
preceding exon, thus creating a recursive 5′ splice site (RS-5ss). Previous studies focused …

The exon junction complex: a lifelong guardian of mRNA fate

LA Woodward, JW Mabin, P Gangras… - Wiley Interdisciplinary …, 2017 - Wiley Online Library
During messenger RNA (mRNA) biogenesis and processing in the nucleus, many proteins
are imprinted on mRNAs assembling them into messenger ribonucleoproteins (mRNPs) …

Imaging of endogenous messenger RNA splice variants in living cells reveals nuclear retention of transcripts inaccessible to nonsense-mediated decay in Arabidopsis

J Göhring, J Jacak, A Barta - The Plant Cell, 2014 - academic.oup.com
Alternative splicing (AS) is an important regulatory process that leads to the creation of
multiple RNA transcripts from a single gene. Alternative transcripts often carry premature …

Insights into the design and interpretation of iCLIP experiments

N Haberman, I Huppertz, J Attig, J König, Z Wang… - Genome biology, 2017 - Springer
Background Ultraviolet (UV) crosslinking and immunoprecipitation (CLIP) identifies the sites
on RNAs that are in direct contact with RNA-binding proteins (RBPs). Several variants of …

[HTML][HTML] CWC22 connects pre-mRNA splicing and exon junction complex assembly

AL Steckelberg, V Boehm, AM Gromadzka, NH Gehring - Cell reports, 2012 - cell.com
The exon junction complex (EJC) is a key regulator of posttranscriptional mRNA fate and
binds to mRNA during splicing. Although the composition of EJCs is well understood, the …