The Bacterial Enhancer-Dependent ς54(ςN) Transcription Factor
M Buck, MT Gallegos, DJ Studholme, Y Guo… - Journal of …, 2000 - Am Soc Microbiol
The initiation of transcription is a complex process involving many different steps. These
steps are all potential control points for regulating gene expression, and many have been …
steps are all potential control points for regulating gene expression, and many have been …
Regulation of Type VI Secretion Gene Clusters by σ54 and Cognate Enhancer Binding Proteins
CS Bernard, YR Brunet, M Gavioli, R Lloubes… - Journal of …, 2011 - Am Soc Microbiol
Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines
responsible for the release of at least two proteins in the milieu, which are thought to form an …
responsible for the release of at least two proteins in the milieu, which are thought to form an …
Transcriptional activation via DNA-looping: visualization of intermediates in the activation pathway of E. coli RNA polymerase· σ54 holoenzyme by scanning force …
Scanning force microscopy (SFM) has been used to study transcriptional activation of
Escherichia coli RNA polymerase· σ54 (RNAP· σ54) at the glnA promoter by the constitutive …
Escherichia coli RNA polymerase· σ54 (RNAP· σ54) at the glnA promoter by the constitutive …
[HTML][HTML] A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase
Z Wang, H Wang, N Mulvenna… - Frontiers in …, 2021 - frontiersin.org
DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding
sites of the DNA-binding proteins and deny further access to these sites by DNA. Such …
sites of the DNA-binding proteins and deny further access to these sites by DNA. Such …
Converting Escherichia coli RNA Polymerase into an Enhancer-Responsive Enzyme: Role of an NH2-Terminal Leucine Patch in σ54
JT Wang, A Syed, M Hsieh, JD Gralla - Science, 1995 - science.org
The protein σ54 associates with Escherichia coli core RNA polymerase to form a
holoenzyme that binds promoters but is inactive in the absence of enhancer activation. Here …
holoenzyme that binds promoters but is inactive in the absence of enhancer activation. Here …
The domain structure of sigma 54 as determined by analysis of a set of deletion mutants
C Wong, Y Tintut, JD Gralla - Journal of molecular biology, 1994 - Elsevier
Escherichia coli σ54 was analyzed by making a series of 16 internal deletions within its
gene and analyzing the properties of the mutant proteins. All of the mutant proteins except …
gene and analyzing the properties of the mutant proteins. All of the mutant proteins except …
The RpoN‐box motif of the RNA polymerase sigma factor σN plays a role in promoter recognition
M Taylor, R Butler, S Chambers… - Molecular …, 1996 - Wiley Online Library
The RNA polymerase sigma factor σN (σ54) is characterized by the presence, near the C‐
terminal end of the protein, of a highly conserved sequence of 10 amino acids …
terminal end of the protein, of a highly conserved sequence of 10 amino acids …
[HTML][HTML] A fork junction DNA–protein switch that controls promoter melting by the bacterial enhancer‐dependent sigma factor
Y Guo, L Wang, JD Gralla - The EMBO Journal, 1999 - embopress.org
Results of binding assays using DNA fork junction probes indicate that sigma 54 contains
multiple determinants that regulate melting to allow RNA polymerase to remain in closed …
multiple determinants that regulate melting to allow RNA polymerase to remain in closed …
Structure of the RNA polymerase core-binding domain of σ54 reveals a likely conformational fracture point
E Hong, M Doucleff, DE Wemmer - Journal of molecular biology, 2009 - Elsevier
Transcription initiation by bacterial σ54-RNA polymerase requires a conformational change
of the holopolymerase–DNA complex, driven by an enhancer-binding protein. Although …
of the holopolymerase–DNA complex, driven by an enhancer-binding protein. Although …
Core RNA polymerase and promoter DNA interactions of purified domains of σN: bipartite functions
W Cannon, S Missailidis, C Smith, A Cottier… - Journal of molecular …, 1995 - Elsevier
The σNclass of sigma factors confer upon RNA polymerase the requirement for enhancer-
binding activator proteins. The sigma-N (σN) protein ofKlebsiella pneumoniaewas analysed …
binding activator proteins. The sigma-N (σN) protein ofKlebsiella pneumoniaewas analysed …