A user's guide to golden gate cloning methods and standards
JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …
of applications has driven the development of many different cloning strategies. One method …
Plant synthetic biology innovations for biofuels and bioproducts
Plant-based biosynthesis of fuels, chemicals, and materials promotes environmental
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
TIR domains of plant immune receptors are 2′, 3′-cAMP/cGMP synthetases mediating cell death
Summary 2′, 3′-cAMP is a positional isomer of the well-established second messenger
3′, 5′-cAMP, but little is known about the biology of this noncanonical cyclic nucleotide …
3′, 5′-cAMP, but little is known about the biology of this noncanonical cyclic nucleotide …
Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to
detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 …
detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 …
A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors
Plant nucleotide binding/leucine-rich repeat (NLR) immune receptors are activated by
pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain …
pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain …
EDS1 complexes are not required for PRR responses and execute TNL‐ETI from the nucleus in Nicotiana benthamiana
J Zönnchen, J Gantner, D Lapin, K Barthel… - New …, 2022 - Wiley Online Library
Heterodimeric complexes incorporating the lipase‐like proteins EDS1 with PAD4 or SAG101
are central hubs in plant innate immunity. EDS1 functions encompass signal relay from TIR …
are central hubs in plant innate immunity. EDS1 functions encompass signal relay from TIR …
Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design
DNA assembly is an integral part of modern synthetic biology, as intricate genetic
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana
Heterodimeric complexes containing the lipase-like protein ENHANCED DISEASE
SUSCEPTIBILITY1 (EDS1) are regarded as central regulators of plant innate immunity. In …
SUSCEPTIBILITY1 (EDS1) are regarded as central regulators of plant innate immunity. In …
Highly efficient multiplex editing: one‐shot generation of 8× Nicotiana benthamiana and 12× Arabidopsis mutants
J Stuttmann, K Barthel, P Martin, J Ordon… - The Plant …, 2021 - Wiley Online Library
Genome editing by RNA‐guided nucleases, such as SpCas9, has been used in numerous
different plant species. However, to what extent multiple independent loci can be targeted …
different plant species. However, to what extent multiple independent loci can be targeted …
IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation
Spatiotemporal control of cell division is essential for the growth and development of
multicellular organisms. In plant cells, proper cell plate insertion during cytokinesis relies on …
multicellular organisms. In plant cells, proper cell plate insertion during cytokinesis relies on …