RNA interference (RNAi), the sequence-specific suppression of gene expression, offers
great opportunities for insect science, especially to analyze gene function, manage pest …

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …

Reverse transcription quantitative polymerase chain reaction (qRT-PCR) has rapidly
become the most sensitive and accurate method for the quantification of gene expression …

The brown planthopper (BPH), Nilaparvata lugens (Hemiptera, Delphacidae), is one of the
most important rice pests. Abundant genetic studies on BPH have been conducted using …

Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires
an appropriate normalization strategy to control for variation among samples. The best …

The triatomine Rhodnius prolixus, a vector of the etiological agent of Chagas disease, has
long been used as model to understand important aspects of insect physiology. Despite this …

Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for
measuring and evaluating changes in gene expression. The most common method for …

Rhodnius prolixus is a blood-gorging insect that is medically important since it transmits
Chagas disease via feces and urine that contain the parasite Trypanosoma cruzi. In adult …

To accurately assess gene expression levels, it is essential to normalize real-time
quantitative PCR (RT-qPCR) data with suitable internal reference genes. For the red …

Quantitative real-time polymerase chain reaction (qRT-PCR) is a valuable tool for estimating
gene expression; however, the validity is largely dependent on the selection of stable …