The role of protein loops and linkers in conformational dynamics and allostery

E Papaleo, G Saladino, M Lambrughi… - Chemical …, 2016 - ACS Publications
Proteins are dynamic entities that undergo a plethora of conformational changes that may
take place on a wide range of time scales. These changes can be as small as the rotation of …

Psychrophilic enzymes: from folding to function and biotechnology

G Feller - Scientifica, 2013 - Wiley Online Library
Psychrophiles thriving permanently at near‐zero temperatures synthesize cold‐active
enzymes to sustain their cell cycle. Genome sequences, proteomic, and transcriptomic …

Function and biotechnology of extremophilic enzymes in low water activity

R Karan, MD Capes, S DasSarma - Aquatic biosystems, 2012 - Springer
Enzymes from extremophilic microorganisms usually catalyze chemical reactions in non-
standard conditions. Such conditions promote aggregation, precipitation, and denaturation …

Rebuilding the lid region from conformational and dynamic features to engineering applications of lipase in foods: Current status and future prospects

G Chen, IM Khan, W He, Y Li, P Jin… - … Reviews in Food …, 2022 - Wiley Online Library
The applications of lipases in esterification, amidation, and transesterification have
broadened their potential in the production of fine compounds with high cumulative values …

Computation of enzyme cold adaptation

J Åqvist, GV Isaksen, BO Brandsdal - Nature Reviews Chemistry, 2017 - nature.com
Earth has several environments that are potentially hostile to life. The survival of organisms
has required the expression of proteins that are adapted to function under extreme …

An optimal distance cutoff for contact-based Protein Structure Networks using side-chain centers of mass

J Salamanca Viloria, MF Allega, M Lambrughi… - Scientific reports, 2017 - nature.com
Proteins are highly dynamic entities attaining a myriad of different conformations. Protein
side chains change their states during dynamics, causing clashes that are propagated at …

Biotechnology of cold-active proteases

S Joshi, T Satyanarayana - Biology, 2013 - mdpi.com
The bulk of Earth's biosphere is cold (< 5° C) and inhabited by psychrophiles. Biocatalysts
from psychrophilic organisms (psychrozymes) have attracted attention because of their …

Computer simulations explain the anomalous temperature optimum in a cold-adapted enzyme

J Sočan, M Purg, J Åqvist - Nature communications, 2020 - nature.com
Cold-adapted enzymes from psychrophilic species show the general characteristics of being
more heat labile, and having a different balance between enthalpic and entropic …

Comparing mutagenesis and simulations as tools for identifying functionally important sequence changes for protein thermal adaptation

M Liao, GN Somero, Y Dong - Proceedings of the National …, 2019 - National Acad Sciences
Comparative studies of orthologous proteins of species evolved at different temperatures
have revealed consistent patterns of temperature-related variation in thermal stabilities of …

Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution

R Martinez, F Jakob, R Tu, P Siegert… - Biotechnology and …, 2013 - Wiley Online Library
Abstract Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease
exhibiting activity and stability properties suitable for applications in laundry and dish …