Process for protein PEGylation

D Pfister, M Morbidelli - Journal of Controlled Release, 2014 - Elsevier
PEGylation is a versatile drug delivery technique that presents a particularly wide range of
conjugation chemistry and polymer structure. The conjugated protein can be tuned to …

[HTML][HTML] Liquid biphasic system: A recent bioseparation technology

KS Khoo, HY Leong, KW Chew, JW Lim, TC Ling… - Processes, 2020 - mdpi.com
A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted
many researchers' interest for being an alternative bioseparation technology for various …

[图书][B] Laboratory techniques in biochemistry and molecular biology

TR Flotte, KI Berns - 1969 - books.google.com
Adeno-associated viruses (AAV) are ubiquitous in the human population (> 90% of adults
are seropositive) but have never been implicated as the cause of any disease. They were …

PEG-proteins: reaction engineering and separation issues

CJ Fee, JM Van Alstine - Chemical engineering science, 2006 - Elsevier
Poly (ethylene glycol)-conjugated (or PEGylated) proteins are an increasingly important
class of therapeutic proteins that offer improved in vivo circulation half lives over their …

[HTML][HTML] Antibody fragments and their purification by protein L affinity chromatography

G Rodrigo, M Gruvegård, JM Van Alstine - Antibodies, 2015 - mdpi.com
Antibodies and related proteins comprise one of the largest and fastest-growing classes of
protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however …

Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Partitioning in aqueous two-phase systems: recent results

H Walter, G Johansson, DE Brooks - Analytical biochemistry, 1991 - Elsevier
From analytical to commercial scale, aqueous twophase systems have a niche in the
purification, characterization, and study of biomaterials (1). Initially described by Beijerinck …

Synthesis and Purification of Thermally Sensitive Oligomer−Enzyme Conjugates of Poly(N-isopropylacrylamide)−Trypsin

Z Ding, G Chen, AS Hoffman - Bioconjugate chemistry, 1996 - ACS Publications
Using chain-transfer polymerization, we have synthesized oligomers of poly (N-
isopropylacrylamide)[poly (NIPAAm)] with one carboxyl group at the end of each oligomer …

Affinity-enhanced purification of human antibodies by aqueous two-phase extraction

AM Azevedo, PAJ Rosa, IF Ferreira, A Pisco… - Separation and …, 2009 - Elsevier
The partition of human antibodies in polyethylene glycol (PEG)/dextran aqueous two-phase
systems (ATPS) was investigated using charged, hydrophobic and affinity ligands, either in …

Virus bioresistor (VBR) for Detection of bladder cancer marker DJ-1 in urine at 10 pM in one minute

A Bhasin, EC Sanders, JM Ziegler, JS Briggs… - Analytical …, 2020 - ACS Publications
DJ-1, a 20.7 kDa protein, is overexpressed in people who have bladder cancer (BC). Its
elevated concentration in urine allows it to serve as a marker for BC. However, no biosensor …