CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Sequence modeling and design from molecular to genome scale with Evo
The genome is a sequence that encodes the DNA, RNA, and proteins that orchestrate an
organism's function. We present Evo, a long-context genomic foundation model with a …
organism's function. We present Evo, a long-context genomic foundation model with a …
Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
TnpB structure reveals minimal functional core of Cas12 nuclease family
G Sasnauskas, G Tamulaitiene, G Druteika, A Carabias… - Nature, 2023 - nature.com
The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
Structural basis of Gabija anti-phage defence and viral immune evasion
SP Antine, AG Johnson, SE Mooney, A Leavitt… - Nature, 2024 - nature.com
Bacteria encode hundreds of diverse defence systems that protect them from viral infection
and inhibit phage propagation,,,–. Gabija is one of the most prevalent anti-phage defence …
and inhibit phage propagation,,,–. Gabija is one of the most prevalent anti-phage defence …
Improved genome editing by an engineered CRISPR-Cas12a
CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …