CRISPR technology: A decade of genome editing is only the beginning

JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

CRISPR technologies for genome, epigenome and transcriptome editing

L Villiger, J Joung, L Koblan, J Weissman… - … Reviews Molecular Cell …, 2024 - nature.com
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Sequence modeling and design from molecular to genome scale with Evo

E Nguyen, M Poli, MG Durrant, B Kang, D Katrekar… - Science, 2024 - science.org
The genome is a sequence that encodes the DNA, RNA, and proteins that orchestrate an
organism's function. We present Evo, a long-context genomic foundation model with a …

Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors

B Al-Shayeb, P Skopintsev, KM Soczek, EC Stahl, Z Li… - Cell, 2022 - cell.com
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria

DC Volke, E Orsi, PI Nikel - Current Opinion in Microbiology, 2023 - Elsevier
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

TnpB structure reveals minimal functional core of Cas12 nuclease family

G Sasnauskas, G Tamulaitiene, G Druteika, A Carabias… - Nature, 2023 - nature.com
The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …

Structural basis of Gabija anti-phage defence and viral immune evasion

SP Antine, AG Johnson, SE Mooney, A Leavitt… - Nature, 2024 - nature.com
Bacteria encode hundreds of diverse defence systems that protect them from viral infection
and inhibit phage propagation,,,–. Gabija is one of the most prevalent anti-phage defence …

Improved genome editing by an engineered CRISPR-Cas12a

E Ma, K Chen, H Shi, EC Stahl, B Adler… - Nucleic Acids …, 2022 - academic.oup.com
CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …

Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox

EV Koonin, JS Gootenberg, OO Abudayyeh - Biochemistry, 2023 - ACS Publications
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …