Chemical synthesis of bioactive proteins
O Harel, M Jbara - Angewandte Chemie, 2023 - Wiley Online Library
Nature has developed a plethora of protein machinery to operate and maintain nearly every
task of cellular life. These processes are tightly regulated via post‐expression modifications …
task of cellular life. These processes are tightly regulated via post‐expression modifications …
Synthesis of ubiquitinated proteins for biochemical and functional analysis
J Kriegesmann, A Brik - Chemical Science, 2023 - pubs.rsc.org
Ubiquitination plays a crucial role in controlling various biological processes such as
translation, DNA repair and immune response. Protein degradation for example, is one of …
translation, DNA repair and immune response. Protein degradation for example, is one of …
Chemoselective Caging of Carboxyl Groups for On‐Demand Protein Activation with Small Molecules
YD Petri, CS Gutierrez… - Angewandte Chemie …, 2023 - Wiley Online Library
Tools for on‐demand protein activation enable impactful gain‐of‐function studies in
biological settings. Thus far, however, proteins have been chemically caged at primarily Lys …
biological settings. Thus far, however, proteins have been chemically caged at primarily Lys …
Site-Specific Acetylation of the Transcription Factor Protein Max Modulates Its DNA Binding Activity
RV Nithun, YM Yao, O Harel, S Habiballah… - ACS Central …, 2024 - ACS Publications
Chemical protein synthesis provides a powerful means to prepare novel modified proteins
with precision down to the atomic level, enabling an unprecedented opportunity to …
with precision down to the atomic level, enabling an unprecedented opportunity to …
Suspension Bead Loading (SBL): An Economical Protein Delivery Platform to Study URM1's Behavior in Live Cells
Uniquely modified synthetic proteins are difficult to produce in large quantities, which could
limit their use in various in vitro settings and in cellular studies. In this study, we developed a …
limit their use in various in vitro settings and in cellular studies. In this study, we developed a …
Chemical protein synthesis combined with protein cell delivery reveals new insights on the maturation process of SUMO2
D Shkolnik, S Dey, M Hasan, MJ Matunis, A Brik - Chemical Science, 2025 - pubs.rsc.org
The Small Ubiquitin-like Modifier (SUMO) is a crucial post-translational modifier of proteins,
playing a key role in various cellular functions. All SUMOs are synthesized as precursor …
playing a key role in various cellular functions. All SUMOs are synthesized as precursor …
Wash-free Imaging in Live Cells
M Hasan, A Brik - 2025 - ACS Publications
Wash-free Imaging in Live Cells | ACS Central Science Recently Viewedclose modal ACS
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ACS Publications C&EN CAS Find my institution Log In ACS Publications. Most Trusted. Most …
De novo Semi‐Synthetic Platform for Monitoring Protein degradation in Live Cells
We designed a platform for monitoring the degradation of exogenous proteins in live cells.
We engineered a semi‐synthetic platform, which consists of Enhanced Green Fluorescent …
We engineered a semi‐synthetic platform, which consists of Enhanced Green Fluorescent …
Semisynthesis reveals apoptin as a tumour-selective protein prodrug that causes cytoskeletal collapse
J Wyatt, YK Chan, M Hess, M Tavassoli… - Chemical Science, 2023 - pubs.rsc.org
Apoptin is a small viral protein capable of inducing cell death selectively in cancer cells.
Despite its potential as an anticancer agent, relatively little is known about its mechanism of …
Despite its potential as an anticancer agent, relatively little is known about its mechanism of …
Protein Delivery and Mimicry
Ü Langel - CPP, Cell-Penetrating Peptides, 2023 - Springer
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