Transposon insertion sequencing (TIS) is a powerful approach that can be extensively
applied to the genome-wide definition of loci that are required for bacterial growth under …

Measuring biological data across time and space is critical for understanding complex
biological processes and for various biosurveillance applications. However, such data are …

The microbial cell wall is essential for maintenance of cell shape and resistance to external
stressors. The primary structural component of the cell wall is peptidoglycan, a glycopolymer …

Infection with Mycobacterium tuberculosis causes a spectrum of outcomes; the majority of
individuals contain but do not eliminate the infection, while a small subset present with …

Streptococcus pneumoniae is an opportunistic human pathogen that causes invasive
diseases, including pneumonia, with greater health risks upon influenza A virus (IAV) co …

Host-associated bacterial communities vary extensively between individuals, but it can be
very difficult to determine the sources of this heterogeneity. Here, we demonstrate that …

Summary Future Challenges in Plant Pathology Plant infection is a complicated process. On
encountering a plant, pathogenic microorganisms must first adapt to life on the epiphytic …

The metaphor of a bottleneck has been used in a variety of fields to describe the critical
constraints that limit a system's performance or capacity. In biology, particularly in studies of …

Vibrio parahaemolyticus is the most common cause of seafood-borne gastroenteritis
worldwide and a blight on global aquaculture. This organism requires a horizontally …

DNA-amplicon-based microbiota profiling can estimate species diversity and abundance but
cannot resolve genetic differences within individuals of the same species. Here we report …