The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology

C Schmitt-Engel, D Schultheis, J Schwirz… - Nature …, 2015 - nature.com
Genetic screens are powerful tools to identify the genes required for a given biological
process. However, for technical reasons, comprehensive screens have been restricted to …

How two extraembryonic epithelia became one: serosa and amnion features and functions of Drosophila's amnioserosa

U Schmidt-Ott, CW Kwan - Philosophical Transactions of …, 2022 - royalsocietypublishing.org
The conservation of gene networks that specify and differentiate distinct tissues has long
been a subject of great interest to evolutionary developmental biologists, but the question of …

Morphogenetic functions of extraembryonic membranes in insects

U Schmidt-Ott, CW Kwan - Current opinion in insect science, 2016 - Elsevier
Highlights•Amnioserosa cells are functionally and evolutionarily comparable to amnion
cells.•Amnioserosa cells regulate dorsal closure through controlled osmosis and …

Co-option of stress mechanisms in the origin of evolutionary novelties

AC Love, GP Wagner - Evolution, 2022 - academic.oup.com
It is widely accepted that stressful conditions can facilitate evolutionary change. The
mechanisms elucidated thus far accomplish this with a generic increase in heritable …

Non-invasive long-term fluorescence live imaging of Tribolium castaneum embryos

F Strobl, EHK Stelzer - Development, 2014 - journals.biologists.com
Insect development has contributed significantly to our understanding of metazoan
development. However, most information has been obtained by analyzing a single species …

The beetle amnion and serosa functionally interact as apposed epithelia

M Hilbrant, T Horn, S Koelzer, KA Panfilio - Elife, 2016 - elifesciences.org
Unlike passive rupture of the human chorioamnion at birth, the insect extraembryonic (EE)
tissues–the amnion and serosa–actively rupture and withdraw in late embryogenesis …

Persistent Parental RNAi in the Beetle Tribolium castaneum Involves Maternal Transmission of Long Double‐Stranded RNA

T Horn, KD Narov, KA Panfilio - Advanced Genetics, 2022 - Wiley Online Library
Parental RNA interference (pRNAi) is a powerful and widely used method for gene‐specific
knockdown. Yet in insects its efficacy varies between species, and how the systemic …

Evolution and multiple roles of the Pancrustacea specific transcription factor zelda in insects

L Ribeiro, V Tobias-Santos, D Santos, F Antunes… - PLoS …, 2017 - journals.plos.org
Gene regulatory networks (GRNs) evolve as a result of the coevolutionary processes acting
on transcription factors (TFs) and the cis-regulatory modules they bind. The zinc-finger TF …

Live imaging of Tribolium castaneum embryonic development using light-sheet–based fluorescence microscopy

F Strobl, A Schmitz, EHK Stelzer - Nature protocols, 2015 - nature.com
Tribolium castaneum has become an important insect model organism for evolutionary
developmental biology, genetics and biotechnology. However, few protocols for live …

Feeding‐structure morphogenesis in “rhabditid” and diplogastrid nematodes is not controlled by a conserved genetic module

T Theska, RJ Sommer - Evolution & Development, 2024 - Wiley Online Library
Disentangling the evolution of the molecular processes and genetic networks that facilitate
the emergence of morphological novelties is one of the main objectives in evolutionary …