Fluorescence correlation spectroscopy (FCS) is becoming increasingly popular as a
technique that aims at complementing live cell images with biophysical information. This …

Fluorescence correlation spectroscopy (FCS) extracts information about molecular dynamics
from the tiny fluctuations that can be observed in the emission of small ensembles of …

Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for
quantifying protein-protein interactions,,,,. In this technique, two different fluorescent labels …

Dual-color fluorescence cross-correlation spectroscopy (FCCS) allows for the determination
of molecular mobility and concentrations and for the quantitative analysis of molecular …

Nucleosomes are multi-component macromolecular assemblies which present a formidable
obstacle to enzymatic activities that require access to the DNA, eg DNA and RNA …

Molecular diffusion in biological membranes is a determining factor in cell signaling and cell
function. In the past few decades, three main fluorescence spectroscopy techniques have …

Although methods for light microscopy of chromatin are well established, there are no
quantitative data for nucleosome concentrations in vivo. To establish such a method we …

Assembly of the Gag polyprotein into new viral particles in infected cells is a crucial step in
the retroviral replication cycle. Currently, little is known about the onset of assembly in the …

Here we discuss the application of scanning fluorescence correlation spectroscopy (SFCS)
using continuous wave excitation to analyze membrane dynamics. The high count rate per …

Movement of particles in cell nuclei can be affected by viscosity, directed flows, active
transport, or the presence of obstacles such as the chromatin network. Here we investigate …