Isothermal titration calorimetry (ITC) is a fast, accurate and label‐free method for measuring
the thermodynamics and binding affinities of molecular associations in solution. Because the …

Most of the implant-associated infections are attributed to bacteria adhering to biomaterial
surfaces as “biofilm” communities. Bacterial transport, first contact with the surface as well as …

Advances in synthetic biology allow the design and manipulation of DNA from the scale of
genes to genomes, enabling the engineering of complex genetic information for application …

Bacterial transformation is a crucial part of cloning process and has been widely used in
many studies (Swords, 2003; Gigova et al., 2006). The mechanism is marked by two phases …

Antibiotic resistance genes (ARGs) in bacteria are emerging contaminants as their
proliferation in the environment poses significant threats to human health. It is well …

Various parameters of standard CaCl2/heat shock method on transformation of Escherichia
coli strain DH5α-T1 R with plasmid pUC19 were optimized. Of the four different heat shock …

Homologous recombination is the most complex of all recombination events that shape
genomes and produce material for evolution. Homologous recombination events are …

Escherichia coli is not assumed to be naturally transformable. However, several recent
reports have shown that E. coli can express modest genetic competence in certain …

The efficiencies of different transformation methods of E. coli DH5α strain, induced by
several cations like Mg2+, Mn2+, Rb+ and especially Ca2+, with or without polyethylene …

Double zwitterionic amino acid Schiff base, o-vanillylidene-l-histidine (OVHIS) and its
copper complex (CuOVHIS) have been synthesized and characterized. CuOVHIS has …