Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

It is well established that cells sense chemical signals from their local microenvironment and
transduce them to the nucleus to regulate gene expression programmes. Although a number …

Eukaryotic genomes are packaged into a 3-dimensional structure in the nucleus. Current
methods for studying genome-wide structure are based on proximity ligation. However, this …

Three-dimensional genome structures play a key role in gene regulation and cell functions.
Characterization of genome structures necessitates single-cell measurements. This has …

Visualization of the transcriptome and the nuclear organization in situ has been challenging
for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method …

There is increasing evidence that both mechanical and biochemical signals play important
roles in development and disease. The development of complex organisms, in particular …

The nucleus is linked mechanically to the extracellular matrix via multiple polymers that
transmit forces to the nuclear envelope and into the nuclear interior. Here, we review some …

Cells in tissues experience a plethora of forces that regulate their fate and modulate
development and homeostasis. Cells sense mechanical cues through localized …

Cellular behavior is continuously affected by microenvironmental forces through the process
of mechanotransduction, in which mechanical stimuli are rapidly converted to biochemical …

Cells are constantly subjected to a spectrum of mechanical cues, such as shear stress,
compression, differential tissue rigidity and strain, to which they adapt by engaging …