State-of-the-art fluorescence fluctuation-based spectroscopic techniques for the study of protein aggregation

A Kitamura, M Kinjo - International journal of molecular sciences, 2018 - mdpi.com
Neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Alzheimer's
disease, Parkinson's disease, and Huntington's disease, are devastating proteinopathies …

Identification of agents targeting FtsZ assembly

D Panda, D Bhattacharya, QH Gao… - Future medicinal …, 2016 - Taylor & Francis
Filamenting temperature-sensitive mutant Z (FtsZ), an essential cell division protein in
bacteria, has recently emerged as an important and exploitable antibacterial target …

Fluorescence and scattering light cross correlation spectroscopy and its applications in homogeneous immunoassay

J Wang, X Huang, H Liu, C Dong, J Ren - Analytical chemistry, 2017 - ACS Publications
In this work, we propose fluorescence and scattering light cross-correlation spectroscopy
(FSCCS) based on laser confocal configuration using silver nanoparticle (SNPs) and Alexa …

Filamentous temperature sensitive mutant Z: a putative target to combat antibacterial resistance

S Kifayat, V Yele, A Ashames, DK Sigalapalli… - RSC …, 2023 - pubs.rsc.org
In the pre-antibiotic era, common bacterial infections accounted for high mortality and
morbidity. Moreover, the discovery of penicillin in 1928 marked the beginning of an antibiotic …

FtsZ interactions and biomolecular condensates as potential targets for new antibiotics

S Zorrilla, B Monterroso, MÁ Robles-Ramos… - Antibiotics, 2021 - mdpi.com
FtsZ is an essential and central protein for cell division in most bacteria. Because of its ability
to organize into dynamic polymers at the cell membrane and recruit other protein partners to …

A mechanism of salt bridge–mediated resistance to FtsZ inhibitor PC190723 revealed by a cell-based screen

AK Sharma, SM Poddar, J Chakraborty… - Molecular biology of …, 2023 - Am Soc Cell Biol
Bacterial cell division proteins, especially the tubulin homologue FtsZ, have emerged as
strong targets for developing new antibiotics. Here, we have utilized the fission yeast …

Detection of substrate binding of a collagen-specific molecular chaperone HSP47 in solution using fluorescence correlation spectroscopy

A Kitamura, Y Ishida, H Kubota, CG Pack… - Biochemical and …, 2018 - Elsevier
Heat shock protein 47 kDa (HSP47), an ER-resident and collagen-specific molecular
chaperone, recognizes collagenous hydrophobic amino acid sequences (Gly-Pro-Hyp) and …

Relationship Between Homodimeric Glucocorticoid Receptor and Transcriptional Regulation Assessed via an In Vitro Fluorescence Correlation Spectroscopy …

S Oasa, S Mikuni, J Yamamoto, T Kurosaki… - Scientific Reports, 2018 - nature.com
Glucocorticoid receptor (GR) is a hormone-activated transcription regulatory protein involved
in metabolism as well as adrenocortical responses to psychosocial stress. Ligand-activated …

[HTML][HTML] A Highlights from MBoC Selection: A mechanism of salt bridge–mediated resistance to FtsZ inhibitor PC190723 revealed by a cell-based screen

AK Sharma, SM Poddar, J Chakraborty… - Molecular Biology of …, 2023 - ncbi.nlm.nih.gov
Bacterial cell division proteins, especially the tubulin homologue FtsZ, have emerged as
strong targets for developing new antibiotics. Here, we have utilized the fission yeast …

Non-stoichiometric inhibition in integrated lead finding–a literature review

M Klumpp - Expert opinion on drug discovery, 2016 - Taylor & Francis
Introduction: Non-stoichiometric inhibition summarizes different mechanisms by which low-
molecular weight compounds can reproducibly inhibit high-throughput screening (HTS) and …