Microfluidics-based strategies for molecular diagnostics of infectious diseases

X Wang, XZ Hong, YW Li, Y Li, J Wang, P Chen… - Military Medical …, 2022 - Springer
Traditional diagnostic strategies for infectious disease detection require benchtop
instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a …

[HTML][HTML] A roadmap to high-speed polymerase chain reaction (PCR): COVID-19 as a technology accelerator

M Madadelahi, R Agarwal, SO Martinez-Chapa… - Biosensors and …, 2024 - Elsevier
The limit of detection (LOD), speed, and cost of crucial COVID-19 diagnostic tools, including
lateral flow assays (LFA), enzyme-linked immunosorbent assays (ELISA), and polymerase …

A review on microscale polymerase chain reaction based methods in molecular diagnosis, and future prospects for the fabrication of fully integrated portable …

NY Lee - Microchimica Acta, 2018 - Springer
Since the advent of microfabrication technology and soft lithography, the lab-on-a-chip
concept has emerged as a state-of-the-art miniaturized tool for conducting the multiple …

Functional nucleic acid lateral flow magnetic biosensor based on blocking the super PCR and magnetic test strip for rapid detection of genetically modified maize …

S Li, J Tian, L Zhu, K Huang, H Chu, W Xu - Analytica Chimica Acta, 2022 - Elsevier
Although PCR is a typical temperature-variable amplification technique for nucleic acids, it
still faces challenges in rapid screening, such as low speed, poor sensitivity, inferior …

Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip

J Ji, C Hu, X Pang, J Liang, Q Huang, S Hu, Q Mei… - ACS …, 2024 - ACS Publications
In this paper, a digital microfluidic thermal control system was introduced for the stable
polymerase chain reaction (PCR). The system consists of a thermoelectric cooler unit, a …

Localized air-mediated heating method for isothermal and rapid thermal processing on lab-on-a-disk platforms

E Pishbin, M Eghbal, M Navidbakhsh… - Sensors and Actuators B …, 2019 - Elsevier
In this study, an air-mediated local heating method is developed for Lab-on-a-Disk platforms.
The hot air is blown on a rotating disk through a dual-sided nozzle for heating up the specific …

Deskilled and rapid drug-resistant gene detection by centrifugal force-assisted thermal convection PCR device

WV Espulgar, M Saito, K Takahashi, S Ushiro… - Sensors, 2021 - mdpi.com
Here we report the improved Cyclo olefin polymer (COP) microfluidic chip and polymerase
chain reaction (PCR) amplification system for point-of-care testing (POCT) in rapid detection …

Analysis of Separation Efficiency Focusing on Particle Concentration and Size Using a Spiral Microfluidic Device

M Horade, S Mukae, T Yamawaki… - Journal of Robotics …, 2023 - jstage.jst.go.jp
This study discusses component separation using a microfluidic device. Based on the
separation principle, a method was adopted to generate an external force due to centrifugal …

Utility of centrifugation-controlled convective (C3) flow for rapid on-chip ELISA

W Espulgar, T Tadokoro, E Tamiya, M Saito - Scientific Reports, 2019 - nature.com
Miniaturizing the enzyme-linked immunosorbent assay (ELISA) protocols in microfluidics is
sought after by researchers for a rapid, high throughput screening, on-site diagnosis, and …

Biocomputing label-free security system based on homogenous ligation chain reaction-induced dramatic change in melting temperature for screening single …

C Xue, X Yu, S Hu, M Luo, Z Shen, P Yuan, ZS Wu - Talanta, 2020 - Elsevier
The development of smart platform with accurate, inexpensive and reliable detection of
single-nucleotide polymorphisms (SNPs) has long been concerned in the fields of medical …