For about three decades, the chemistry of cation radicals derived from organic molecules
had received a major impetus from mass spectrometry. This was because cation radicals …

Despite major advantages in the field of proteomics, the analysis of PTMs still poses a major
challenge; thus far, preventing insights into the role and regulation of protein networks …

Electron-based dissociation (ExD) produces uncluttered mass spectra of intact proteins
while preserving labile post-translational modifications. However, technical challenges have …

We introduce a method for sequencing peptides by mass spectrometry using a
metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When …

“Native” mass spectrometry (MS) has been proven to be increasingly useful for structural
biology studies of macromolecular assemblies. Using horse liver alcohol dehydrogenase …

The extensive analytical characterization of protein biotherapeutics, especially of
biosimilars, is a critical part of the product development and registration. High-resolution …

An efficient approach to easy and reliable differentiation between isomeric leucine and
isoleucine in peptide sequencing utilizes multistage electron transfer dissociation and …

The fragmentation chemistry of peptides containing intrachain disulfide bonds was
investigated under electron transfer dissociation (ETD) conditions. Fragments within the …

An EThcD-based approach for the reliable discrimination of isomeric leucine and isoleucine
residues in peptide de novo sequencing procedure has been proposed. A multistage …

Aim To identify the different antifungal biomolecules produced by isolate Bacillus vallismortis
R2 and analyse their effect on Alternaria alternata, a common pathogen causing black point …