[PDF][PDF] Aqueous two phase systems for the recovery of biomolecules–a review

S Raja, VR Murty, V Thivaharan, V Rajasekar… - Sci Technol, 2011 - academia.edu
Aqueous two phase system (ATPS) is a liquid–liquid extraction method which employs two
aqueous phases having applications in the field of biotechnology for the separation and …

Aqueous two-phase systems based on ionic liquids and deep eutectic solvents as a tool for the recovery of non-protein bioactive compounds—a review

FS Buarque, GV Gautério, MAZ Coelho, AC Lemes… - Processes, 2022 - mdpi.com
Aqueous two-phase systems (ATPS) based on ionic liquids (IL) and deep eutectic solvents
(DES) are ecofriendly choices and can be used to selectively separate compounds of …

Extraction and downstream processing of plant-derived recombinant proteins

JF Buyel, RM Twyman, R Fischer - Biotechnology advances, 2015 - Elsevier
Plants offer the tantalizing prospect of low-cost automated manufacturing processes for
biopharmaceutical proteins, but several challenges must be addressed before such goals …

[PDF][PDF] Aqueous two-phase extraction advances for bioseparation

AM Goja, H Yang, M Cui, C Li - J. Bioprocess. Biotechnol, 2013 - pdfs.semanticscholar.org
Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of
solute from one aqueous phase to another. ATPE includes polymer–polymer type and …

Aqueous two-phase systems based on acetonitrile and carbohydrates and their application to the extraction of vanillin

G de Brito Cardoso, T Mourão, FM Pereira… - Separation and …, 2013 - Elsevier
Aqueous two-phase systems (ATPS) are an important technique for the extraction and
purification of biomolecules. In aqueous media, many pairs of solutes can be used to …

Continuous aqueous two-phase systems devices for the recovery of biological products

E Espitia-Saloma, P Vázquez-Villegas, O Aguilar… - Food and Bioproducts …, 2014 - Elsevier
Aqueous two-phase systems (ATPS) have proved to be a suitable technique for the recovery
of biological products. Although ATPS have been in the field of primary recovery and …

Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro

P Wan, Z Sheng, Q Han, Y Zhao, G Cheng… - Journal of Chromatography …, 2014 - Elsevier
Enrichment and purification of total flavonoids from Flos Populi extracts were studied using
five macroporous resins. The static tests indicated that NKA-9 resin was appropriate and its …

Process development strategies in plant molecular farming

JF Buyel - Current Pharmaceutical Biotechnology, 2015 - ingentaconnect.com
Protein-based biopharmaceuticals are often produced in mammalian cell cultures, which are
more expensive than microbial systems but capable of authentic post-translational …

Continuous purification of an enveloped and non-enveloped viral particle using an aqueous two-phase system

DG Turpeinen, PU Joshi, SA Kriz, S Kaur… - Separation and …, 2021 - Elsevier
Meeting the increasing demand for vaccines throughout the world is key to decrease the
spread of infectious diseases. The switch to a fully continuous vaccine manufacturing …

Optimization of PEG–salt aqueous two-phase systems by design of experiments

A Glyk, D Solle, T Scheper, S Beutel - Chemometrics and Intelligent …, 2015 - Elsevier
Since the mechanism governing the partitioning behavior of biomolecules, such as proteins
and enzymes, in polyethylene glycol (PEG)–salt aqueous two-phase systems (ATPS) is …