The yeast Saccharomyces cerevisiae has been a favorite organism for pioneering studies
on nutrient-sensing and signaling mechanisms. Many specific nutrient responses have been …

Transcription factors are adaptor molecules that detect regulatory sequences in the DNA
and target the assembly of protein complexes that control gene expression. Yet much of the …

A major goal of biology is to provide a quantitative description of cellular behaviour. This
task, however, has been hampered by the difficulty in measuring protein abundances and …

DNA segments that actively regulate transcription in vivo are typically characterized by
eviction of nucleosomes from chromatin and are experimentally identified by their …

Understanding the mechanisms by which chromatin structure controls eukaryotic
transcription has been an intense area of investigation for the past 25 years. Many of the key …

The identification of nuclease-hypersensitive sites in an active globin gene and in the 5′
regions of fruit fly heat shock genes first suggested that chromatin changes accompany gene …

The DNA of eukaryotic genomes is wrapped in nucleosomes, which strongly distort and
occlude the DNA from access to most DNA-binding proteins. An understanding of the …

Recent reports reinforce the notion that nucleosomes are highly dynamic in response to the
process of transcription. Nucleosomes are displaced at promoters during gene activation in …

Background Although eukaryotic genomes are generally thought to be entirely chromatin-
associated, the activated PHO5 promoter in yeast is largely devoid of nucleosomes. We …

Promoters play an essential role in the regulation of gene expression for fine-tuning genetic
circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However …